1887

Abstract

The significance of the gene product on chemotrophic sulfur oxidation of was investigated. The thioredoxin SoxS was purified, and the N-terminal amino acid sequence identified SoxS as the gene product. The wild-type formed thiosulfate-oxidizing activity and Sox proteins during mixotrophic growth with succinate plus thiosulfate, while there was no activity, and only traces of Sox proteins, under heterotrophic conditions. The homogenote mutant strain GBΩS is unable to express the genes, of which encodes a transcriptional regulator. Strain GBΩS cultivated mixotrophically showed about 22 % of the specific thiosulfate-dependent O uptake rate of the wild-type, and when cultivated heterotrophically it produced 35 % activity. However, under both mixotrophic and heterotrophic conditions, strain GBΩS formed Sox proteins essential for sulfur oxidation at the same high level as the wild-type produced them during mixotrophic growth. Genetic complementation of strain GBΩS with restored the activity upon mixotrophic and heterotrophic growth. Chemical complementation by reductants such as -cysteine, DTT and tris(2-carboxyethyl)phosphine also restored the activity of strain GBΩS in the presence of chloramphenicol, which is an inhibitor of protein synthesis. The data demonstrate that SoxS plays a key role in activation of the Sox enzyme system, and this suggests that SoxS is part of a novel type of redox control in .

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2007-04-01
2019-11-13
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