1887

Abstract

Active extracellular carbonic anhydrases (CAs) were found in the alkaliphilic stromatolite-forming cyanobacterium . Enzyme activity was detected in intact cells and in the cell envelope fraction. Western blot analysis of polypeptides from the cell envelope suggested the presence of at least two polypeptides cross-reacting with antibodies against both and classes of CA. Immunocytochemical analysis revealed putative -CA localized in the glycocalyx. This -CA has a molecular mass of about 34 kDa and a pI of 3.5. External CAs showed two peaks of activity at around pH 10 and 7.5. The possible involvement of extracellular CAs of in photosynthetic assimilation of inorganic carbon and its relationship to CaCO deposition during mineralization of cyanobacterial cells are discussed.

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2007-04-01
2020-04-09
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References

  1. Arp G., Reimer A., Reitner J.. 2001; Photosynthesis-induced biofilm calcification and calcium concentrations in phanerozoic oceans. Science292:1701–1704[CrossRef]
    [Google Scholar]
  2. Badger M. R., Price G. D.. 2003; CO2 concentrating mechanisms in cyanobacteria: molecular components, their diversity and evolution. J Exp Bot54:609–622[CrossRef]
    [Google Scholar]
  3. Balnokin Y. V., Popova L. G., Pagis L. Y., Andreev I. M.. 2004; The Na+-translocating ATPase in the plasma membrane of the marine microalga Tetraselmis viridis catalyzes Na+/H+ exchange. Planta219:332–337[CrossRef]
    [Google Scholar]
  4. Castenholz R. W., Waterbury J. B.. 1989; Group I. Cyanobacteria. In Bergey's Manual of Systematic Bacteriology vol3 pp1710–1727 Edited by Staley J. T.. Bryant M. P., Pfennig N., Holt J. G.. Baltimore, MD: Williams & Wilkins;
    [Google Scholar]
  5. Chirica L. C., Elleby B., Jonsson B. H., Lindskog S.. 1997; The complete sequence, expression in Escherichia coli , purification and some properties of carbonic anhydrase from Neisseria gonorrhoeae. Eur J Biochem244:755–760[CrossRef]
    [Google Scholar]
  6. Dudoladova M. V., Markelova A. G., Lebedeva N. V., Pronina N. A.. 2004; Compartmentation of α - and β -carbonic anhydrases in the cells of halo- and alkaliphilic cyanobacterium Rhabdoderma lineare. Russ J Plant Physiol51:806–814[CrossRef]
    [Google Scholar]
  7. Eriksson M., Karlsson J., Ramazanov Z., Gardestrom P., Samuelsson G.. 1996; Discovery of an algal mitochondrial carbonic anhydrase: molecular cloning and characterisation of a low-CO2-induced polypeptide in Chlamydomonas reinhardtii. Proc Natl Acad Sci U S A93:12031–12034[CrossRef]
    [Google Scholar]
  8. Fawcett T. W., Browse J. A., Volokita M., Bartlett S. G.. 1990; Spinach carbonic anhydrase primary structure deduced from the sequence of a cDNA clone. J Biol Chem265:5414–5417
    [Google Scholar]
  9. Gantt E.. 1994; Supermolecular membrane organization. In The Molecular Biology of Cyanobacteria pp119–138 Edited by Bryant D. A.. Dordrecht: Kluwer;
    [Google Scholar]
  10. Gerasimenko L. M., Mityushina L. L., Namsaraev B. B.. 2003; Microcoleus mats from alkaliphilic and halophilic communities. Mikrobiologiia72:71–79
    [Google Scholar]
  11. Hiltonen T., Bjorkbacka H., Forsman C., Clarke A. K., Samuelsson G.. 1998; Intracellular β -carbonic anhydrase of the unicellular green alga Coccomyxa . Cloning of the cDNA and characterization of the functional enzyme overexpressed in Escherichia coli. Plant Physiol117:1341–1349[CrossRef]
    [Google Scholar]
  12. Huang F., Hedman E., Funk C., Kieselbach T., Schroder W. P., Norling B.. 2004; Isolation of outer membrane of Synechocystis sp. PCC 6803 and its proteomic characterization. Mol Cell Proteomics3:586–595[CrossRef]
    [Google Scholar]
  13. Karlsson J., Clarke A. K., Chen Z. Y., Hugghins S. Y., Park Y.-I., Husic H. D., Moroney J. V., Samuelsson G.. 1998; A novel alpha-type carbonic anhydrase associated with the thylakoid membrane in Chlamydomonas reinhardtii is required at ambient CO2. EMBO J17:1208–1216[CrossRef]
    [Google Scholar]
  14. Katzman G. L., Carlson S. J., Marcus Y., Moroney J. V., Togasaki R. K.. 1994; Carbonic anhydrase activity in isolated chloroplasts of wild-type and high-CO2-dependent mutants of Chlamydomonas reinhardtii as studied by a new assay. Plant Physiol105:1197–1202
    [Google Scholar]
  15. Kupriyanova E. V., Lebedeva N. V., Dudoladova M. V., Gerasimenko L. M., Alekseeva S. G., Pronina N. A., Zavarzin G. A.. 2003; Carbonic anhydrase activity of alkaliphilic cyanobacteria from soda lakes. Russ J Plant Physiol50:532–539[CrossRef]
    [Google Scholar]
  16. Laemmli U. K.. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227:680–685[CrossRef]
    [Google Scholar]
  17. McConnaughey T. A.. 1994; Calcification, photosynthesis and global carbon cycles. Bull Inst Oceanogr13:137–161
    [Google Scholar]
  18. Nafi B. M., Miles R. J., Butler L. O., Carter N. D., Kelly C., Jeffery S.. 1990; Expression of carbonic anhydrase in neisseriae and other heterotrophic bacteria. J Med Microbiol32:1–7[CrossRef]
    [Google Scholar]
  19. Nishiyama Y., Yamamoto H., Allakhverdiev S. I., Inaba M., Yokota A., Murata N.. 2001; Oxidative stress inhibits the repair of photodamage to the photosynthetic machinery. EMBO J20:5587–5594[CrossRef]
    [Google Scholar]
  20. Park Y.-I., Karlsson J., Rojdestvenski I., Pronina N., Klimov V., Oquist G., Samuelsson G.. 1999; Role of a novel photosystem II-associated carbonic anhydrase in photosynthetic carbon assimilation in Chlamydomonas reinhardtii. FEBS Lett444:102–105[CrossRef]
    [Google Scholar]
  21. Porra R. J., Thompson W. A., Kriedemann P. E.. 1989; Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentrations of chlorophyll standards by absorption spectroscopy. Biochim Biophys Acta975:384–394[CrossRef]
    [Google Scholar]
  22. Quiroga O., Gonzalez E. L.. 1993; Carbonic anhydrase in the chloroplast of a coccolithophorid (Prymnesiophyceae. J Phycol29:321–324[CrossRef]
    [Google Scholar]
  23. Rippka R., DeReuelles J., Waterbury J. B., Herdman M., Stanier R. Y.. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol111:1–61[CrossRef]
    [Google Scholar]
  24. Sergeev V. N., Gerasimenko L. M., Zavarzin G. A.. 2002; The proterozoic history and present state of cyanobacteria. Mikrobiologiia71:623–637
    [Google Scholar]
  25. Smith K. S., Ferry J. G.. 2000; Prokaryotic carbonic anhydrases. FEMS Microbiol Rev24:335–366[CrossRef]
    [Google Scholar]
  26. So A. K., Van Spall H. G. C., Coleman J. R., Espie O. S.. 1998; Catalytic exchange of 18O from 13C18O-labelled CO2 by wild type cells and ecaA , ecaB , and ccaA mutants of the cyanobacteria Synechococcus PCC 7942 and Synechocystis PCC 6803. Can J Bot76:1153–1160
    [Google Scholar]
  27. So A. K., Espie G. S., Williams E. B., Shively J. M., Heinhorst S., Cannon G. C.. 2004; A novel evolutionary lineage of carbonic anhydrase (epsilon class) is a component of the carboxysome shell. J Bacteriol186:623–630[CrossRef]
    [Google Scholar]
  28. Soltes-Rak E., Mulligan M. E., Coleman J. R.. 1997; Identification and characterization of gene encoding a vertebrate-type carbonic anhydrase in cyanobacteria. J Bacteriol179:769–774
    [Google Scholar]
  29. Tripp B. C., Smith K., Ferry J. G.. 2001; Carbonic anhydrase: New insights for an ancient enzyme. J Biol Chem276:48615–48618[CrossRef]
    [Google Scholar]
  30. Villarejo A., Shutova T., Moskvin O., Forssen M., Klimov V. V., Samuelsson G.. 2002; A photosystem II-associated carbonic anhydrase regulates the efficiency of photosynthetic oxygen evolution. EMBO J21:1930–1938[CrossRef]
    [Google Scholar]
  31. Weckesser J., Jürgens U. J.. 1988; Cell walls and external layers. Methods Enzymol167:173–188
    [Google Scholar]
  32. Wilbur K. M., Anderson N. G.. 1948; Electrometric and colorimetric determination of carbonic anhydrase. J Biol Chem176:147–154
    [Google Scholar]
  33. Yagawa Y., Shiraiwa Y., Miyachi S.. 1984; Carbonic anhydrase from the blue-green alga (cyanobacterium) Anabaena variabilis. Plant Cell Physiol25:775–783
    [Google Scholar]
  34. Zavarzin G. A.. 2002; Microbial geochemical calcium cycle. Mikrobiologiia71:1–17
    [Google Scholar]
  35. Zavarzin G. A., Orleanskii V. K., Gerasimenko L. M., Pushko S. N., Ushatinskaya G. T.. 2003; Laboratory simulations of cyanobacterial mats of the alkaline geochemical barrier. Mikrobiologiia72:80–85
    [Google Scholar]
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