1887

Abstract

biotype 3 has been implicated as the causative pathogen of an ongoing disease outbreak that erupted in Israel in 1996. Recent work based on multi-locus sequence typing (MLST) showed that biotype 3 is genetically homogeneous. The aim of this study was to investigate the existence of subpopulations within this homogeneous biotype by characterizing the surface antigens and analysing the sequence diversity of selected outer-membrane protein (OMP)-encoding genes. Rabbit antisera were prepared against biotype 1, 2 and 3 strains. The results of the slide-agglutination test, dot-blot assay (using fresh and boiled cells), and immunoblotting of lipopolysaccharides (LPS) and OMPs were evaluated. By slide-agglutination and dot-blot assays all biotype 3 strains agglutinated with the selected biotype 3 strain. This homogeneity was supported by immunoblot analysis of the LPS. Analysis of OMP patterns revealed that all three biotypes share a considerable number of common bands that are antigenically related. Cluster analysis of DNA sequence data from selected OMP-encoding genes showed that biotype 3 strains form a genetically distinct and homogeneous clone. The homogeneity of surface antigens and the lack of any sequence diversity among both housekeeping and OMP-encoding genes reaffirms the highly clonal nature of biotype 3 and suggests that it has only recently descended from the parent population of .

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2007-03-01
2019-08-23
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References

  1. Amaro, C., Biosca, E. G., Fouz, B. & Garay, E. ( 1992a; ). Electrophoretic analysis of heterogeneous lipopolysaccharides from various strains of Vibrio vulnificus biotypes 1 and 2 by silver staining and immunoblotting. Curr Microbiol 25, 99–104.[CrossRef]
    [Google Scholar]
  2. Amaro, C., Biosca, E. G., Esteve, C., Fouz, B. & Toranzo, A. E. ( 1992b; ). Comparative study of phenotypic and virulence properties in Vibrio vulnficus biotype 1 and 2 obtained from a European eel farm experiencing mortalities. Dis Aquat Organ 13, 29–35.[CrossRef]
    [Google Scholar]
  3. Amaro, C., Fouz, B., Collado, R., Esteve-Gassent, D., Marcelo-Noales, E., Esteve, C., Barrera, R. & Alcaide, E. ( 2001; ). Design and development of a vaccine against eel vibriosis caused by Vibrio vulnificus serovar E (or biotype 2) applied to intensive culture facilities (Report). Valencia: Departamento de Microbiologia y Ecologia, Universidad de Valencia, Valencia, Spain.
  4. Biosca, E. G., Garay, E., Toranzo, A. E. & Amaro, C. ( 1993; ). Comparison of outer membrane protein profiles of Vibrio vulnificus biotypes 1 and 2. FEMS Microbiol Lett 107, 217–222.[CrossRef]
    [Google Scholar]
  5. Biosca, E. G., Oliver, J. D. & Amaro, C. ( 1996; ). Phenotypic characterization of Vibrio vulnificus biotype 2, a lipopolysaccharide-based homogeneous O serogroup within Vibrio vulnificus. Appl Environ Microbiol 62, 918–927.
    [Google Scholar]
  6. Biosca, E. G., Marco-Noales, E., Amaro, C. & Alcaide, E. ( 1997; ). An enzyme-linked immunosorbent assay for detection of Vibrio vulnificus biotype 2: development and field studies. Appl Environ Microbiol 63, 537–542.
    [Google Scholar]
  7. Bisharat, N. & Raz, R. ( 1996; ). Vibrio infection in Israel due to changes in fish marketing. Lancet 348, 1585–1586.
    [Google Scholar]
  8. Bisharat, N., Agmon, V., Finkelstein, R., Raz, R., Ben-Dror, G., Lerner, L., Soboh, S., Colodner, R., Cameron, D. N. & other authors ( 1999; ). Clinical, epidemiological, and microbiological features of Vibrio vulnificus biogroup 3 causing outbreaks of wound infection and bacteraemia in Israel. Israel Vibrio Study Group. Lancet 354, 1421–1424.[CrossRef]
    [Google Scholar]
  9. Bisharat, N., Cohen, D. I., Harding, R. M., Falush, D., Crook, D. W., Peto, T. & Maiden, M. C. ( 2005; ). Hybrid Vibrio vulnificus. Emerg Infect Dis 11, 30–35.[CrossRef]
    [Google Scholar]
  10. Blake, P. A., Merson, M. H., Weaver, R. E., Hollis, D. G. & Heublein, P. C. ( 1979; ). Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med 300, 1–5.[CrossRef]
    [Google Scholar]
  11. Chen, C. Y., Wu, K. M., Chang, Y. C., Chang, C. H., Tsai, H. C., Liao, T. L., Liu, Y. M., Chen, H. J., Shen, A. B. & other authors ( 2003; ). Comparative genome analysis of Vibrio vulnificus, a marine pathogen. Genome Res 13, 2577–2587.[CrossRef]
    [Google Scholar]
  12. Cipriano, R. C., Pyle, J. B., Starliper, C. E. & Pyle, S. W. ( 1985; ). Detection of Vibrio anguillarum antigen by the dot blot assay. J Wildl Dis 21, 211–218.[CrossRef]
    [Google Scholar]
  13. Collado, R., Fouz, B., Sanjuan, E. & Amaro, C. ( 2000; ). Effectiveness of different vaccine formulations against vibriosis caused by Vibrio vulnificus serovar E (biotype 2) in European eels Anguilla anguilla. Dis Aquat Organ 43, 91–101.[CrossRef]
    [Google Scholar]
  14. Cox, A., Zou, W., Gidney, M. A., Lacelle, S., Plested, J. S., Makepeace, K., Wright, J. C., Coull, P. A., Moxon, E. R. & Richards, J. C. ( 2005; ). Candidacy of LPS-based glycoconjugates to prevent invasive meningococcal disease: developmental chemistry and investigation of immunological responses following immunization of mice and rabbits. Vaccine 23, 5045–5054.[CrossRef]
    [Google Scholar]
  15. Dalsgaard, A., Frimodt-Moller, N., Bruun, B., Hoi, L. & Larsen, J. L. ( 1996; ). Clinical manifestations and molecular epidemiology of Vibrio vulnificus infections in Denmark. Eur J Clin Microbiol Infect Dis 15, 227–232.[CrossRef]
    [Google Scholar]
  16. Esteve-Gassent, M. D. & Amaro, C. ( 2004; ). Immunogenic antigens of the eel pathogen Vibrio vulnificus serovar E. Fish Shellfish Immunol 17, 277–291.[CrossRef]
    [Google Scholar]
  17. Fukuda, Y. & Kusuda, R. ( 1985; ). Vaccination of Yellowtail against pseudotuberculosis. Fish Pathol 20, 421–425.[CrossRef]
    [Google Scholar]
  18. Gutacker, M., Conza, N., Benagli, C., Pedroli, A., Bernasconi, M. V., Permin, L., Aznar, R. & Piffaretti, J. C. ( 2003; ). Population genetics of Vibrio vulnificus: identification of two divisions and a distinct eel-pathogenic clone. Appl Environ Microbiol 69, 3203–3212.[CrossRef]
    [Google Scholar]
  19. Hitchcock, P. J. & Brown, T. M. ( 1983; ). Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154, 269–277.
    [Google Scholar]
  20. Jolley, K. A., Feil, E. J., Chan, M. S. & Maiden, M. C. ( 2001; ). Sequence type analysis and recombinational tests (START). Bioinformatics 17, 1230–1231.[CrossRef]
    [Google Scholar]
  21. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  22. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685.[CrossRef]
    [Google Scholar]
  23. Maiden, M. C., Bygraves, J. A., Feil, E., Morelli, G., Russell, J. E., Unwin, R., Zhang, Q., Zhou, J., Zurth, K. & other authors ( 1998; ). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 95, 3140–3145.[CrossRef]
    [Google Scholar]
  24. Oliver, J. D. ( 1989; ). Foodborne Bacterial Pathogens, pp. 569–600. Edited by M. P. Doyle. New York: Marcel Dekker.
  25. Oliver, J. D. & Colwell, R. R. ( 1973; ). Extractable lipids of gram-negative marine bacteria: phospholipid composition. J Bacteriol 114, 897–908.
    [Google Scholar]
  26. Park, D. K., Lee, K. E., Baek, C. H., Kim, I. H., Kwon, J. H., Lee, W. K., Lee, K. H., Kim, B. S., Choi, S. H. & Kim, K. S. ( 2006; ). Cyclo(Phe-Pro) modulates the expression of ompU in Vibrio spp. J Bacteriol 188, 2214–2221.[CrossRef]
    [Google Scholar]
  27. Parmley, S. F., Gross, U., Sucharczuk, A., Windeck, T., Sgarlato, G. D. & Remington, J. S. ( 1994; ). Two alleles of the gene encoding surface antigen P22 in 25 strains of Toxoplasma gondii. J Parasitol 80, 293–301.[CrossRef]
    [Google Scholar]
  28. Paz, S., Bisharat, N., Paz, E., Kidar, O. & Cohen, D. ( 2006; ). Climate change and the emergence of Vibrio vulnificus disease in Israel. Environ Res (in press) http://www.sciencedirect.com/science/journal/00139351.
    [Google Scholar]
  29. Provenzano, D., Lauriano, C. M. & Klose, K. E. ( 2001; ). Characterization of the role of the ToxR-modulated outer membrane porins OmpU and OmpT in Vibrio cholerae virulence. J Bacteriol 183, 3652–3662.[CrossRef]
    [Google Scholar]
  30. Renia, L., Ling, I. T., Marussig, M., Miltgen, F., Holder, A. A. & Mazier, D. ( 1997; ). Immunization with a recombinant C-terminal fragment of Plasmodium yoelii merozoite surface protein 1 protects mice against homologous but not heterologous P. yoelii sporozoite challenge. Infect Immun 65, 4419–4423.
    [Google Scholar]
  31. Salati, F. & Kusuda, R. ( 1985; ). Vaccine preparations used for immunization of eel Anguilla japonica against Edwarsiella tarda infection. Bull Jpn Soc Sci Fish 51, 1233–1237.[CrossRef]
    [Google Scholar]
  32. Smith, A. B. & Siebeling, R. J. ( 2003; ). Identification of genetic loci required for capsular expression in Vibrio vulnificus. Infect Immun 71, 1091–1097.[CrossRef]
    [Google Scholar]
  33. Smith, J. M., Smith, N. H., O'Rourke, M. & Spratt, B. G. ( 1993; ). How clonal are bacteria? Proc Natl Acad Sci U S A 90, 4384–4388.[CrossRef]
    [Google Scholar]
  34. Sorensen, U. B. & Larsen, J. L. ( 1986; ). Serotyping of Vibrio anguillarum. Appl Environ Microbiol 51, 593–597.
    [Google Scholar]
  35. Staden, R. ( 1996; ). The Staden sequence analysis package. Mol Biotechnol 5, 233–241.[CrossRef]
    [Google Scholar]
  36. Tison, D. L., Nishibuchi, M., Greenwood, J. D. & Seidler, R. J. ( 1982; ). Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl Environ Microbiol 44, 640–646.
    [Google Scholar]
  37. Towbin, H., Staehelin, T. & Gordon, J. ( 1979; ). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A 76, 4350–4354.[CrossRef]
    [Google Scholar]
  38. Webster, A. C. & Litwin, C. M. ( 2000; ). Cloning and characterization of vuuA, a gene encoding the Vibrio vulnificus ferric vulnibactin receptor. Infect Immun 68, 526–534.[CrossRef]
    [Google Scholar]
  39. Wright, A. C., Powell, J. L., Kaper, J. B. & Morris, J. G., Jr ( 2001; ). Identification of a group 1-like capsular polysaccharide operon for Vibrio vulnificus. Infect Immun 69, 6893–6901.[CrossRef]
    [Google Scholar]
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