1887

Abstract

A previous microarray analysis suggested that multicopy , encoding a function-unknown response regulator, enhances expression of , which encodes a two-gene ATP-binding cassette transporter involved in the extrusion of sodium ions. The two-component regulatory system YccG–YccH was therefore renamed NatK–NatR. Here, this observation was confirmed by a fusion analysis using a strain carrying . Further, in both and mutants, expression was completely abolished, indicating that the NatK–NatR system positively regulates the expression of . In a gel retardation analysis, NatR bound to the promoter region. Using purified His-tagged NatR, DNase I footprinting analysis of the promoter region suggested that a direct repeat of [TTCA(G)CGACA], separated by a 12 bp space, would be recognized by NatR. Deleted and mutagenized promoter regions of were analysed using a fusion, and it was confirmed that the direct repeat is critical for activation by NatR.

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2007-03-01
2019-10-13
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vol. , part 3, pp. 667-675

Structure of plasmid pDG-N17. Boxes and the angled arrow labelled P5 represent genes and an IPTG-inducible T5 promoter, respectively. The I and HI cloning sites are shown. Ap , Em and Sp indicate the ampicillin, erythromycin and spectinomycin resistance genes, respectively.



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