1887

Abstract

Display of heterologous proteins on the surface of micro-organisms has become an increasingly used strategy for a range of applications in microbiology, biotechnology and vaccinology. In this study, the potential of the major structural protein DraE of Dr fimbriae as a display system for heterologous sequences was tested. One copy of a heterologous sequence mimicking a small P epitope of simian virus 5 was inserted into the gene, replacing the N-terminal region of the surface-exposed domain 2 as previously done with the glycoprotein D of herpes simplex virus type 1. The exposure of chimeric proteins on the bacterial surface was detected by immunofluorescence microscopy. Insertion of the heterogenic peptides had no detectable effect on the Ig-barrel structure of the DraE fimbrial subunits, as confirmed by FTIR spectroscopy. Additionally, the affinity of the chimeric fimbriae for DAF and type IV collagen was similar to that of the wild-type Dr fimbriae.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2006/003525-0
2007-08-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/8/2733.html?itemId=/content/journal/micro/10.1099/mic.0.2006/003525-0&mimeType=html&fmt=ahah

References

  1. Anderson, K. L., Billington, J., Pettigrew, D., Cota, E., Simpson, P., Roversi, P., Chen, H. A., Urvil, P., du Merle, L. & other authors ( 2004; ). An atomic resolution model for assembly, architecture, and function of the Dr adhesins. Mol Cell 15, 647–657.[CrossRef]
    [Google Scholar]
  2. Arrondo, J. L. & Goni, F. M. ( 1999; ). Structure and dynamics of membrane proteins as studied by infrared spectroscopy. Prog Biophys Mol Biol 72, 367–405.[CrossRef]
    [Google Scholar]
  3. Carnoy, C. & Moseley, S. L. ( 1997; ). Mutational analysis of receptor binding mediated by the Dr family of Escherichia coli adhesins. Mol Microbiol 23, 365–379.[CrossRef]
    [Google Scholar]
  4. Das, M., Hart-Van Tassell, A., Urvil, P. T., Lea, S., Pettigrew, D., Anderson, K. L., Samet, A., Kur, J., Matthews, S. & other authors ( 2005; ). Hydrophilic domain II of Escherichia coli Dr fimbriae facilitates cell invasion. Infect Immun 73, 6119–6126.[CrossRef]
    [Google Scholar]
  5. Emini, E. A., Hughes, J. V., Perlow, D. S. & Boger, J. ( 1985; ). Induction of hepatitis A virus-neutralizing antibody by a virus-specific synthetic peptide. J Virol 55, 836–839.
    [Google Scholar]
  6. Georgiou, G., Stathopoulos, C., Daugherty, P. S., Nayak, A. R., Iverson, B. L. & Curtiss, R., III ( 1997; ). Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. Nat Biotechnol 15, 29–34.[CrossRef]
    [Google Scholar]
  7. Giron, J. A., Jones, T., Millan-Velasco, F., Castro-Munoz, E., Zarate, L., Fry, J., Frankel, G., Moseley, S. L., Baudry, B. & Kaper, J. B. ( 1991; ). Diffuse-adhering Escherichia coli (DAEC) as a putative cause of diarrhea in Mayan children in Mexico. J Infect Dis 163, 507–513.[CrossRef]
    [Google Scholar]
  8. Goluszko, P., Goluszko, E., Nowicki, B., Nowicki, S., Popov, V. & Wang, H. Q. ( 2005; ). Vaccination with purified Dr fimbriae reduces mortality associated with chronic urinary tract infection due to Escherichia coli bearing Dr adhesin. Infect Immun 73, 627–631.[CrossRef]
    [Google Scholar]
  9. Haris, P. I. & Severcan, F. ( 1999; ). FTIR spectroscopic characterization of protein structure in aqueous and non-aqueous media. J Mol Catal, B Enzym 7, 207–221.[CrossRef]
    [Google Scholar]
  10. Johnson, J. R. ( 1991; ). Virulence factors in Escherichia coli urinary tract infection. Clin Microbiol Rev 4, 80–128.
    [Google Scholar]
  11. Klemm, P. & Krogfelt, K. A. ( 1994; ). Type 1 fimbriae of Escherichia coli. In Fimbriae, Adhesion, Genetics, Biogenesis and Vaccines, pp. 9–28. Edited by P. Klemm. Boca Raton, FL: CRC Press.
  12. Klemm, P. & Schembri, M. A. ( 2000; ). Fimbriae-assisted bacterial surface display of heterologous peptides. Int J Med Microbiol 290, 215–221.[CrossRef]
    [Google Scholar]
  13. Korotkowa, N., Cota, E., Lebedin, Y., Monpouet, S., Guignot, J., Servin, A. L., Matthews, S. & Moseley, S. L. ( 2006; ). A subfamily of Dr adhesins of Escherichia coli bind independently to decay-accelerating factor and the N-domain of carcinoembryonic antigen. J Biol Chem 281, 29120–29130.[CrossRef]
    [Google Scholar]
  14. Krogfelt, K. A. ( 1991; ). Bacterial adhesion: genetics, biogenesis, and role in pathogenesis of fimbrial adhesins of Escherichia coli. Rev Infect Dis 13, 721–735.[CrossRef]
    [Google Scholar]
  15. Lea, S., Powell, R. & Evans, D. ( 1999; ). Crystallization and preliminary X-ray diffraction analysis of a biologically active fragment of CD55. Acta Crystallogr D Biol Crystallogr 55, 1198–1200.[CrossRef]
    [Google Scholar]
  16. Leclerc, C., Lo-Man, R., Charbit, A., Martineau, P., Clement, J. M. & Hofnung, M. ( 1994a; ). Immunogenicity of viral B- and T-cell epitopes expressed in recombinant bacterial proteins. Int Rev Immunol 11, 123–132.[CrossRef]
    [Google Scholar]
  17. Leclerc, C., Lo-Man, R., Fayolle, C., Charbit, A., Clement, J. M., Martineau, P., O'Callaghan, D. & Hofnung, M. ( 1994b; ). Molecular and cellular parameters controlling the immunogenicity of foreign B- or T-cell epitopes expressed by recombinant vectors. Dev Biol Stand 82, 193–199.
    [Google Scholar]
  18. Levine, M. M., Ferreccio, C., Prado, V., Cayazzo, M., Abrego, P., Martinez, J., Maggi, L., Baldini, M. M., Martin, W. & Maneval, D. ( 1993; ). Epidemiologic studies of Escherichia coli diarrheal infections in a low socioeconomic level peri-urban community in Santiago, Chile. Am J Epidemiol 138, 849–869.
    [Google Scholar]
  19. Moon, H. W. & Bunn, T. O. ( 1993; ). Vaccines for preventing enterotoxigenic Escherichia coli infections in farm animals. Vaccine 11, 213–219.[CrossRef]
    [Google Scholar]
  20. Nowicki, B., Hart, A., Coyne, K. E., Lublin, D. M. & Nowicki, S. ( 1993; ). Short consensus repeat-3 domain of recombinant decay-accelerating factor is recognized by Escherichia coli recombinant Dr adhesin in a model of a cell-cell interaction. J Exp Med 178, 2115–2121.[CrossRef]
    [Google Scholar]
  21. Nowicki, B., Martens, M., Hart, A. & Nowicki, S. ( 1994; ). Gestational age-dependent distribution of Escherichia coli fimbriae in pregnant patients with pyelonephritis. Ann N Y Acad Sci 730, 290–291.[CrossRef]
    [Google Scholar]
  22. Nowicki, B., Selvarangan, R. & Nowicki, S. ( 2001; ). Family of Escherichia coli Dr adhesins: decay-accelerating factor receptor recognition and invasiveness. J Infect Dis (Suppl. 1) 183, S24–S27.[CrossRef]
    [Google Scholar]
  23. Pettigrew, D., Anderson, K. L., Billington, J., Cota, E., Simpson, P., Urvil, P., Rabuzin, F., Roversi, P., Nowicki, B. & other authors ( 2004; ). High resolution studies of the Afa/Dr adhesin DraE and its interaction with chloramphenicol. J Biol Chem 279, 46851–46857.[CrossRef]
    [Google Scholar]
  24. Pham, T. Q., Goluszko, P., Popov, V., Nowicki, S. & Nowicki, B. ( 1997; ). Molecular cloning and characterization of Dr-II, a nonfimbrial adhesin-I-like adhesin isolated from gestational pyelonephritis-associated Escherichia coli that binds to decay-accelerating factor. Infect Immun 65, 4309–4318.
    [Google Scholar]
  25. Selvarangan, R., Goluszko, P., Singhal, J., Carnoy, C., Moseley, S., Hudson, B., Nowicki, S. & Nowicki, B. ( 2004; ). Interaction of Dr adhesin with collagen type IV is a critical step in Escherichia coli renal persistence. Infect Immun 72, 4827–4835.[CrossRef]
    [Google Scholar]
  26. Stentebjerg-Olesen, B., Pallesen, L., Jensen, L. B., Christiansen, G. & Klemm, P. ( 1997; ). Authentic display of a cholera toxin epitope by chimeric type 1 fimbriae: effects of insert position and host background. Microbiology 143, 2027–2038.[CrossRef]
    [Google Scholar]
  27. Subirade, M., Gueguen, J. & Pezolet, M. ( 1994; ). Conformational changes upon dissociation of a globular protein from pea: a Fourier transform infrared spectroscopy study. Biochim Biophys Acta 1205, 239–247.[CrossRef]
    [Google Scholar]
  28. Sun, M., Rothermel, T. A., Shuman, L., Aligo, J. A., Xu, S., Lin, Y., Lamb, R. A. & He, B. ( 2004; ). Conserved cysteine-rich domain of paramyxovirus Simian Virus 5 V protein plays an important role in blocking apoptosis. J Virol 78, 5068–5078.[CrossRef]
    [Google Scholar]
  29. Van Loy, C. P., Sokurenko, E. V. & Moseley, S. L. ( 2002; ). The major structural subunits of Dr and F1845 fimbriae are adhesins. Infect Immun 70, 1694–1702.[CrossRef]
    [Google Scholar]
  30. Westerlund, B., Kuusela, P., Risteli, J., Risteli, L., Vartio, T., Rauvala, H., Virkola, R. & Korhonen, T. K. ( 1989; ). The O75X adhesin of uropathogenic Escherichia coli is a type IV collagen-binding protein. Mol Microbiol 3, 329–337.[CrossRef]
    [Google Scholar]
  31. Young, D. F., Chatziandreou, N., He, B., Goodbourn, S., Lamb, R. A. & Randall, R. E. ( 2001; ). Single amino acid substitution in the V protein of Simian Virus 5 differentiates its ability to block interferon signaling in human and murine cells. J Virol 75, 3363–3370.[CrossRef]
    [Google Scholar]
  32. Zalewska, B., Piątek, R., Konopa, G., Nowicki, B., Nowicki, S. & Kur, J. ( 2003; ). Chimeric Dr fimbriae with a Herpes Simplex virus type 1epitope as a model for a recombinant vaccine. Infect Immun 71, 5505–5513.[CrossRef]
    [Google Scholar]
  33. Zavialov, A. V., Kersley, J., Korpela, T., Zav'yalov, V. P., MacIntyre, S. & Knight, S. D. ( 2002; ). Donor strand complementation mechanism in the biogenesis of non-pilus systems. Mol Microbiol 45, 983–995.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2006/003525-0
Loading
/content/journal/micro/10.1099/mic.0.2006/003525-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error