1887

Abstract

Intimin is an outer-membrane adhesin that is essential for colonization of the host gastrointestinal tract by attaching and effacing pathogens including enteropathogenic (EPEC), enterohaemorrhagic (EHEC) and (CR). The N-terminus of intimin from the different strains is highly conserved while the C-terminus, which harnesses the active receptor-binding site, shows sequence and antigenic polymorphism. This diversity was used to define a number of distinct intimin types, the most common of which are , and . Intimin binds the type III secretion system effector protein Tir. However, a large body of evidence suggests that intimin also binds a host-cell-encoded receptor(s) (Hir), and interaction of different intimin types with Hir contributes to tissue and host specificity. The aims of this study were to compare the activity of the major intimin types (, and ) and , using the CR mouse model and organ culture (IVOC), and to determine their exchangeability. The results confirm that intimin is not functional in the CR mouse model. In the pig, intimin can substitute for EPEC intimin but when placed in an EHEC O157 : H7 background it does not produce an intimin -like tropism, although some adhesion to the small and large intestine was observed. In contrast, in human IVOC, intimin in an EHEC background produces small intestinal colonization in a similar manner to intimin .

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2007-04-01
2019-10-23
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