1887

Abstract

Invasin, the major adhesion and invasion factor of , is encoded by the gene, which is regulated by growth phase and in response to a variety of environmental conditions such as temperature, pH and osmolarity. So far, three proteins, RovA, H-NS and YmoA, have been identified as factors regulating the expression of the gene in enteropathogenic Here, data from  : :  chromosomal gene fusion studies are presented indicating that OmpR, the response regulator of the EnvZ/OmpR two-component system, acts to negatively regulate expression at the transcriptional level at 25 °C, and that high osmolarity enhances the inhibitory effect of this protein. In a strain lacking OmpR the expression of at 25 °C was increased sixfold, but at 37 °C, a temperature known to repress expression, this effect was not observed, suggesting that temperature regulation of is OmpR-independent. Furthermore, the expression of in the background was no longer responsive to increased osmolarity. Complementation with the active allele restored wild-type expression in the mutant. analysis of the O : 9 promoter sequence revealed the presence of an OmpR consensus binding site located in the −15 to −33 region. OmpR was able to specifically bind to a fragment of the promoter containing this putative binding site in electrophoretic mobility shift assays. Thus, OmpR seems to be a repressor of in

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2007-08-01
2020-08-10
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