1887

Abstract

The phytoene-related desaturases are the key enzymes in the carotenoid biosynthetic pathway. The gene encoding phytoene desaturase in the deinoxanthin synthesis pathway of was identified and characterized. Two putative phytoene desaturase homologues (DR0861 and DR0810) were identified by analysis of conserved amino acid regions, and the former displayed the highest identity (68 %) with phytoene desaturase of the cyanobacterium . DR0861 gene knockout and dinucleotide-binding motif deletion resulted in the arrest of lycopene synthesis and the accumulation of phytoene. The colourless DR0861 knockout mutant became more sensitive to acute ionizing radiation and oxygen stress. Complementation of the mutant with a heterologous or homologous gene restored its pigment and resistance. The desaturase activity of DR0861 () was further confirmed by the assay of enzyme activity and heterologous expression in containing and genes (responsible for phytoene synthesis) from . In addition, the amount of lycopene synthesis in resulting from the expression of from was determined, and this had significant dose-dependent effects on the survival rate of exposed to hydrogen peroxide and ionizing radiation.

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2007-05-01
2019-11-12
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