1887

Abstract

The gene of has been shown to play a role in the survival of the avirulent within the macrophage. and analysis of Δ deletion mutants and complemented strains showed no effect on survival of in U-937 macrophages or in a mouse aerosol infection model, respectively. Further studies were done in an attempt to determine the role of in intracellular survival and to define a phenotypic difference between wild-type and the Δ deletion mutant. Bioinformatic analysis indicated that Eis is an acetyltransferase of the GCN5-related family of -acetyltransferases. Immunofluorescence microscopy and Western blot analysis studies demonstrated that Eis is released into the cytoplasm of -infected U-937 macrophages. Eis was also found in the extravesicular fraction and culture supernatant of -infected macrophages. The effect of Eis on human macrophage cytokine secretion was also examined. Eis modulated the secretion of IL-10 and TNF- by primary human monocytes in response both to infection with and to stimulation with recombinant Eis protein. These results suggest that Eis is a mycobacterial effector that is released into the host cell to modulate inflammatory responses, possibly via transcriptional or post-translational means.

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2007-02-01
2020-04-10
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