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Volume 153, Issue 2

Pleiotropic effects of mutations that alter the cytochrome respiratory system Free

Abstract

Using transposon mutagenesis, mutations have been isolated in several genes ( and ) that play a role in cytochrome metabolism. As in other bacteria, mutations in the and genes resulted in the absence of all -type cytochromes. However, the mutant also lacked cytochrome oxidase , a defect that does not appear to have been reported for other bacteria. The -type cytochromes were also missing from a mutant strain with an insertion into the gene encoding the haem-containing subunit (SU)I of cytochrome oxidase, but not in mutants unable to make SUII or SUIII, indicating that CcdA probably plays a role in assembling SUI. The cytochrome-deficient mutants also had other free-living phenotypes, including a significant decrease in growth rate on rich media and increased motility on minimal media. A mutant also had significantly decreased motility, but the motility and growth properties of the mutant were unchanged. Unlike similar mutants in and , an Rm1021 mutant contained cytochrome oxidase . Cytochrome maturation in strain Rm1021 appeared to be similar to maturation in other rhizobia, but there were some differences in the cytochrome composition of the strain, and respiration chain function and assembly.

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Keyword(s): CycM, transmembrane cytochrome c , SU, aa3 cytochrome oxidase subunit and TMPD, N,N,N′,N′-tetramethyl-p-phenylenediamine
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2007-02-01
2025-05-14
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/content/journal/micro/10.1099/mic.0.2006/002634-0
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Pleiotropic effects of mutations that alter the Sinorhizobium meliloti cytochrome c respiratory system
Microbiology 153, 399 (2007); https://doi.org/10.1099/mic.0.2006/002634-0
/content/journal/micro/10.1099/mic.0.2006/002634-0
/content/journal/micro/10.1099/mic.0.2006/002634-0
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