1887

Abstract

Genome-wide data mining indicated that six genes (, , , , and ) encoding putative transport proteins are involved in uptake of various aromatic compounds that are further degraded through the -ketoadipate, gentisate and resorcinol pathways in . The gentisate (GenK/NCgl2922) and vanillate (VanK/NCgl2302) transporters have been identified previously. In this study, physiological functions of the remaining four putative transporters as well as the vanillate transporter (VanK/NCgl2302) were examined by genetic disruption/complementation and uptake assays. Results indicated that encodes PcaK for 4-hydroxybenzoate and protocatechuate transport, and encodes VanK for vanillate transport. Genetic studies and uptake assays indicated that both / and are involved in benzoate transport in . When growth rates were compared for two benzoate transporter mutants, and , a high growth rate was observed for the mutant. Sequence alignments revealed that PcaK, VanK, BenK and GenK belong to the major facilitator superfamily (MFS). Modelling of secondary structures based on previously characterized MFS members revealed that NCgl1031, NCgl2302, NCgl2325 and NCgl2922 are typical 12 helix transmembrane proteins but NCgl2326 contains only 11 -helices. Thus the functionally identified NCgl2326 belongs to a novel type of benzoate transporters. Attempts to identify the phenotype of a mutant failed, so the function of remains unclear.

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2007-03-01
2020-04-10
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