1887

Abstract

The complete genomic sequence of a previously characterized temperate phage of , C2, is reported. The genome is 56 538 bp and organized into 84 putative ORFs in six functional modules. The head and tail structural proteins showed similarities to that of phage CD119 and phage EJ-1, respectively. Homologues of structural and replication proteins were found in prophages 1 and 2 of the sequenced CD630 genome. A putative holin appears unique to the phages and was functional when expressed in . Nucleotide sequence comparisons of C2 to CD119 and the CD630 prophage sequences showed relatedness between C2 and the prophages, but less so to CD119. C2 integrated into a gene encoding a putative transcriptional regulator of the family. C2, CD119 and CD630 prophage 1 genomes had a Cdu1--integrase arrangement, suggesting that the pathogenicity locus (PaLoc) of , flanked by , has phage origins. The sequences of C2, CD119 and CD630 prophages were dissimilar. C2-related sequences were found in 84 % of 37 clinical isolates and typed reference strains.

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2007-03-01
2020-08-13
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