1887

Abstract

Ribosome modulation factor (RMF) was shown to have an influence on the survival of under acid stress during stationary phase, since the viability of cultures of a mutant strain lacking functional RMF decreased more rapidly than that of the parent strain at pH 3. Loss of ribosomes was observed in both strains when exposed to low pH, although this occurred at a higher rate in the RMF-deficient mutant strain, which also suffered from higher levels of rRNA degradation. It was concluded that the action of RMF in limiting the damage to rRNA contributed to the protection of under acid stress. Expression of the gene was lower during stationary phase after growth in acidified media compared to media containing no added acid, and the increased expression associated with transition from exponential phase to stationary phase was much reduced in acidified media. It was demonstrated that RMF was not involved in the stationary-phase acid-tolerance response in by which growth under acidic conditions confers protection against subsequent acid shock. This response was sufficient to overcome the increased vulnerability of the RMF-deficient mutant strain to acid stress at pH values between 6.5 and 5.5.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2006/001552-0
2007-01-01
2019-11-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/1/247.html?itemId=/content/journal/micro/10.1099/mic.0.2006/001552-0&mimeType=html&fmt=ahah

References

  1. Apirakaramwong, A., Fukuchi, J.-I., Kashiwagi, K., Kakinuma, Y., Ito, E., Ishihama, A. & Igarishi, K. ( 1998; ). Enhancement of cell death due to decrease in Mg2+ uptake by OmpC (cation-selective porin) deficiency in ribosome modulation factor-deficient mutant. Biochem Biophys Res Commun 251, 482–487.[CrossRef]
    [Google Scholar]
  2. El-Sharoud, W. M. & Niven, G. W. ( 2005; ). The activity of ribosome modulation factor during growth of Escherichia coli under acidic conditions. Arch Microbiol 184, 18–24.[CrossRef]
    [Google Scholar]
  3. Foster, J. W. ( 1999; ). When protons attack: microbial strategies of acid adaptation. Curr Opin Microbiol 2, 170–174.[CrossRef]
    [Google Scholar]
  4. Foster, J. W. ( 2000; ). Microbial response to acid stress. In Bacterial Stress Responses, pp. 99–115. Edited by G. Storz & R. Hengge-Aronis. Washington, DC: American Society for Microbiology.
  5. Fukuchi, J., Kashiwagi, K., Yamagishi, M., Ishihama, A. & Igarashi, K. ( 1995; ). Decrease in cell viability due to the accumulation of spermidine in spermidine acetyltransferase-deficient mutant of Escherichia coli. J Biol Chem 270, 18831–18835.[CrossRef]
    [Google Scholar]
  6. Garay-Arroyo, A., Colmenero-Flores, J. M., Garciarrubio, A. & Covarrubias, A. A. ( 2000; ). Highly hydrophilic proteins in prokaryotes and eukaryotes are common during conditions of water deficit. J Biol Chem 25, 5668–5674.
    [Google Scholar]
  7. Genthner, F. J. & Martin, S. E. ( 1990; ). Ribosome assembly in Salmonella Typhimurium 7136 during recovery from heat shock. Food Microbiol 7, 53–67.[CrossRef]
    [Google Scholar]
  8. Hayashi, T., Makino, K., Ohnishi, M., Kurokawa, K., Ishii, K., Yokoyama, K., Han, C. G., Ohtsubo, E., Nakayama, K. & other authors ( 2001; ). Complete genome sequence of enterohemorrhagic Escherichia coli O157 : H7 and genomic comparison with a laboratory strain K-12. DNA Res 8, 11–22.[CrossRef]
    [Google Scholar]
  9. Ishihama, A. ( 1999; ). Modulation of the nucleoid, the transcription apparatus, and the translation machinery in bacteria for stationary phase survival. Genes Cells 4, 135–143.[CrossRef]
    [Google Scholar]
  10. Izutsu, K., Wada, A. & Wada, C. ( 2001; ). Expression of ribosome modulation factor (RMF) in Escherichia coli requires ppGpp. Genes Cells 6, 665–676.[CrossRef]
    [Google Scholar]
  11. Kornblum, J. S., Projan, S. J., Moghazeh, S. L. & Novick, R. P. ( 1988; ). A rapid method to quantitate non-labeled RNA species in bacterial cells. Gene 63, 75–85.[CrossRef]
    [Google Scholar]
  12. Maki, Y., Yoshida, H. & Wada, A. ( 2000; ). Two proteins, YfiA and YhbH, associated with resting ribosomes in stationary phase Escherichia coli. Genes Cells 5, 965–974.[CrossRef]
    [Google Scholar]
  13. Miller, J. H. ( 1972; ). Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, NY: Cold Spring Harbor.
  14. Niven, G. W. ( 2004; ). Ribosome modulation factor protects Escherichia coli during heat stress, but this may not be dependent on ribosome dimerisation. Arch Microbiol 162, 60–66.
    [Google Scholar]
  15. Rowbury, R. J. ( 1997; ). Regulatory components, including integration host factor, CysB and H-NS, that influence pH responses in Escherichia coli. Lett Appl Microbiol 24, 319–328.[CrossRef]
    [Google Scholar]
  16. Wada, A. ( 1998; ). Growth phase coupled modulation of Escherichia coli ribosomes. Genes Cells 3, 203–208.[CrossRef]
    [Google Scholar]
  17. Wada, A., Yamazaki, Y., Fujita, N. & Ishihama, A. ( 1990; ). Structure and probable genetic location of a “ribosome modulation factor” associated with 100S ribosomes in stationary-phase Escherichia coli cells. Proc Natl Acad Sci U S A 87, 2657–2661.[CrossRef]
    [Google Scholar]
  18. Wada, A., Mikkola, R., Kurland, C. G. & Ishihama, A. ( 2000; ). Growth phase-coupled changes of the ribosome profile in natural isolates and laboratory strains of Escherichia coli. J Bacteriol 182, 2893–2899.[CrossRef]
    [Google Scholar]
  19. Yamagishi, M., Matsushima, H., Wada, A., Sakagami, M., Fujita, N. & Ishihama, A. ( 1993; ). Regulation of the Escherichia coli rmf gene encoding the ribosome modulation factor – growth phase-dependent and growth rate-dependent control. EMBO J 12, 625–630.
    [Google Scholar]
  20. Yoshida, H., Yamamoto, H., Uchiumi, T. & Wada, A. ( 2004; ). RMF inactivates ribosomes by covering the peptidyl transferase centre and entrance of peptide exit tunnel. Genes Cells 9, 271–278.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2006/001552-0
Loading
/content/journal/micro/10.1099/mic.0.2006/001552-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error