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Volume 153, Issue 3

A novel locus involved in extracellular polysaccharide production and virulence of pathovar Free

Abstract

pathovar () is the causal agent of black rot disease in cruciferous plants. The extracellular polysaccharide (EPS) produced by is an important pathogenicity factor and also has a range of industrial uses. In preliminary work a number of transposon-mediated insertion mutants in with defects in EPS production were identified. Here, one of these mutated loci was investigated in detail. Six ORFs within the locus (ORFs ) were disrupted by plasmid integration. Mutation of , or resulted in significantly reduced EPS production and significantly reduced virulence on the host plant Chinese radish (). The EPS production and virulence of , and mutants could be restored by intact , and genes, respectively, when provided . Although bioinformatic analysis suggested a role for XC3814 and XC3815 in lipopolysaccharide biosynthesis, the lipopolysaccharides produced by the mutants were indistinguishable from those of the wild-type, as judged by electrophoretic mobility in SDS-polyacrylamide gels. These results reveal that , and comprise a novel gene cluster involved in EPS production and virulence of .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): EPS, extracellular polysaccharide and FT-IR, Fourier transform infrared
SGM
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2007-03-01
2024-04-19
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A novel locus involved in extracellular polysaccharide production and virulence of Xanthomonas campestris pathovar campestris
Microbiology 153, 737 (2007); https://doi.org/10.1099/mic.0.2006/001388-0
/content/journal/micro/10.1099/mic.0.2006/001388-0
/content/journal/micro/10.1099/mic.0.2006/001388-0
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