1887

Abstract

Biomining, the use of micro-organisms to recover precious and base metals from mineral ores and concentrates, has developed into a successful and expanding area of biotechnology. While careful considerations are made in the design and engineering of biomining operations, microbiological aspects have been subjected to far less scrutiny and control. Biomining processes employ microbial consortia that are dominated by acidophilic, autotrophic iron- or sulfur-oxidizing prokaryotes. Mineral biooxidation takes place in highly aerated, continuous-flow, stirred-tank reactors or in irrigated dump or heap reactors, both of which provide an open, non-sterile environment. Continuous-flow, stirred tanks are characterized by homogeneous and constant growth conditions where the selection is for rapid growth, and consequently tank consortia tend to be dominated by two or three species of micro-organisms. In contrast, heap reactors provide highly heterogeneous growth environments that change with the age of the heap, and these tend to be colonized by a much greater variety of micro-organisms. Heap micro-organisms grow as biofilms that are not subject to washout and the major challenge is to provide sufficient biodiversity for optimum performance throughout the life of a heap. This review discusses theoretical and pragmatic aspects of assembling microbial consortia to process different mineral ores and concentrates, and the challenges for using constructed consortia in non-sterile industrial-scale operations.

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2007-02-01
2020-08-07
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References

  1. Battaglia-Brunet F., Clarens M., d'Hugues P., Godon J. J., Foucher S., Morin D.. 2002; Monitoring of a pyrite-oxidising bacterial population using DNA single strand conformation polymorphism and microscopic techniques. Appl Microbiol Biotechnol60:206–211[CrossRef]
    [Google Scholar]
  2. Battaglia-Brunet F., Joulian C., Garrido F., Dictor M.-C., Morin D., Coupland K., Johnson D. B., Hallberg K. B., Baranger P.. 2006; Oxidation of arsenite by Thiomonas strains and characterization of Thiomonas arsenivorans sp. nov. Antonie van Leeuwenhoek89:99–108[CrossRef]
    [Google Scholar]
  3. Bruhn D. F., Thompson D. N., Naoh K. S.. 1999; Microbial ecology assessment of a mixed copper oxide/sulfide dump leach operation. In Biohydrometallurgy and the Environment. Toward the Mining of the 21st Century, Process Metallurgy 9A pp799–808 Edited by Amils R., Ballester A.. Amsterdam: Elsevier;
    [Google Scholar]
  4. Clark D. A., Norris P. R.. 1996; Acidimicrobium ferrooxidans gen. nov., sp. nov. mixed culture ferrous iron oxidation with Sulfobacillus species. Microbiology141:785–790
    [Google Scholar]
  5. Coram N. J., Rawlings D. E.. 2002; Molecular relationship between two groups of the genus Leptospirillum and the finding that Leptospirillum ferriphilum sp. nov. dominates South African commercial biooxidation tanks that operate at 40 °C. Appl Environ Microbiol68:838–845[CrossRef]
    [Google Scholar]
  6. Demergasso C. S., Galeguillos P. A., Escudero L. V., Zepeda V. J., Castillo D., Casamayor E. O.. 2005; Molecular characterization of microbial populations in a low-grade copper ore bioleaching test heap. Hydrometallurgy80:241–253[CrossRef]
    [Google Scholar]
  7. Dew D. W., Lawson E. N., Broadhurst J. L.. 1997; The BIOX® process for biooxidation of gold-bearing ores or concentrates. In Biomining: Theory, Microbes and Industrial Processes pp45–80 Edited by Rawlings D. E.. Georgetown, TX: Springer/Landes Bioscience;
    [Google Scholar]
  8. d'Hugues P., Battaglia-Brunet F., Clarens M., Morin D.. 2003; Microbial diversity of various metal-sulphides bioleaching cultures grown under different operating conditions using 16S-rDNA analysis. In Biohydrometallurgy; a Sustainable Technology in Evolution pp1323–1334 Edited by Tsezos M., Hatzikioseyian A., Remoudaki E.. Zografou, Greece: National Technical University of Athens;
    [Google Scholar]
  9. Dopson M., Lindström E. B.. 2004; Analysis of community composition during moderately thermophilic bioleaching of pyrite, arsenical pyrite, and chalcopyrite. Microb Ecol48:19–28[CrossRef]
    [Google Scholar]
  10. Edwards K. J., Hu B., Hamers R. J., Banfield J. F.. 2001; A new look at microbiological leaching patterns on sulfide minerals. FEMS Microbiol Ecol34:197–206[CrossRef]
    [Google Scholar]
  11. Goebel B. M., Stackebrandt E.. 1994; Cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments. Appl Environ Microbiol60:1614–1621
    [Google Scholar]
  12. Golyshina O. V., Pivovarova T. A., Karavaiko G. I., Kondrat'eva T. F., Moore E. R. B., Abraham W. R., Lunsdorf H., Timmis K. N., Yakimov M. M., Golyshin P. N.. 2000; Ferroplasma acidiphilum gen. nov., sp. nov., an acidophilic, autotrophic, ferrous-iron-oxidizing, cell-wall-lacking, mesophilic member of the Ferroplasmaceae fam. nov., comprising a distinct lineage of the Archaea . Int J Syst Evol Microbiol50:997–1006[CrossRef]
    [Google Scholar]
  13. Hallberg K. B., Johnson D. B.. 2001; Biodiversity of acidophilic prokaryotes. Adv Appl Microbiol49:37–84
    [Google Scholar]
  14. Hallberg K. B., Lindström E. B.. 1994; Characterization of Thiobacillus caldus sp. nov., a moderately thermophilic acidophile. Microbiology140:3451–3456[CrossRef]
    [Google Scholar]
  15. Hallberg K. B., Johnson D. B., Williams P. A.. 1999; A novel metabolic phenotype among acidophilic bacteria: aromatic degradation and the potential use of these microorganisms for the treatment of wastewater containing organic and inorganic pollutants. In Biohydrometallurgy and the Environment. Toward the Mining of the 21st Century, Process Metallurgy 9A pp719–728 Edited by Amils R., Ballester A.. Amsterdam: Elsevier;
    [Google Scholar]
  16. Hallberg K. B., Coupland K., Kimura S., Johnson D. B.. 2006; Macroscopic “acid streamer” growths in acidic, metal-rich mine waters in north Wales consist of novel and remarkably simple bacterial communities. Appl Environ Microbiol72:2022–2030[CrossRef]
    [Google Scholar]
  17. Harvey T. J., Bath M.. 2007; The GeoBiotics GEOCOAT technology – progress and challenges. In Biomining pp113–138 Edited by Rawlings D. E., Johnson D. B.. Heidelberg: Springer;
    [Google Scholar]
  18. Hawkes R. B., Franzmann P. D., Plumb J. J.. 2006; Moderate thermophiles including “ Ferroplasma cyprexacervatum ” sp. nov., dominate an industrial scale chalcocite heap bioleaching operation. Hydrometallurgy83:229–236[CrossRef]
    [Google Scholar]
  19. Johnson D. B., Roberto F. F.. 1997; Heterotrophic acidophiles and their roles in the bioleaching of sulfide minerals. In Biomining: Theory, Microbes and Industrial Processes pp259–280 Edited by Rawlings D. E.. Georgetown, TX: Springer/Landes Bioscience;
    [Google Scholar]
  20. Johnson D. B., Rolfe S., Hallberg K. B., Iversen E.. 2001a; Isolation and phylogenetic characterisation of acidophilic microorganisms indigenous to acidic drainage waters at an abandoned Norwegian copper mine. Environ Microbiol3:630–637[CrossRef]
    [Google Scholar]
  21. Johnson D. B., Bacelar-Nicolau P., Okibe N., Yahya A., Hallberg K. B.. 2001b; Role of pure and mixed cultures of Gram-positive eubacteria in mineral leaching. In Biohydrometallurgy: Fundamentals, Technology and Sustainable Development, Process Metallurgy 11A pp461–470 Edited by Ciminelli V. S. T., Garcia O. Jr. Amsterdam: Elsevier;
    [Google Scholar]
  22. Johnson D. B., Okibe N., Roberto F. F.. 2003; Novel thermo-acidophiles isolated from geothermal sites in Yellowstone National Park: physiological and phylogenetic characteristics. Arch Microbiol180:60–68[CrossRef]
    [Google Scholar]
  23. Johnson D. B., Okibe N., Hallberg K. B.. 2005; Differentiation and identification of iron-oxidizing acidophilic bacteria using cultivation techniques and amplified ribosomal DNA restriction enzyme analysis (ARDREA). J Microbiol Methods60:299–313[CrossRef]
    [Google Scholar]
  24. Johnson D. B., Stallwood B., Kimura S., Hallberg K. B.. 2006; Characteristics of Acidicaldus organovorus , gen. nov., sp. nov.; a novel thermo-acidophilic heterotrophic proteobacterium. Arch Microbiol185:212–221[CrossRef]
    [Google Scholar]
  25. Kinnunen H.-M., Puhakka J. A.. 2004; High-rate ferric sulfate generation by a Leptospirillum ferriphilum -dominated biofilm and the role of jarosite in biomass retainment in a fluidized-bed reactor. Biotechnol Bioeng85:697–705[CrossRef]
    [Google Scholar]
  26. Logan T. C., Seal T., Brierley J. A.. 2007; Whole-ore heap biooxidation of sulfidic gold-bearing ores. In Biomining pp113–138 Edited by Rawlings D. E., Johnson D. B.. Heidelberg: Springer;
    [Google Scholar]
  27. Marsh R. M., Norris P. R.. 1983; The isolation of some thermophilic, autotrophic, iron- and sulphur-oxidizing bacteria. FEMS Microbiol Lett17:311–315[CrossRef]
    [Google Scholar]
  28. Mikkelsen D., Kappler U., McEwan A. G., Sly L. I.. 2006; Archaeal diversity in two thermophilic chalcopyrite bioleaching reactors. Environ Microbiol8:2050–2055[CrossRef]
    [Google Scholar]
  29. Norris P. R., Clark D. A., Owen J. P., Waterhouse S.. 1996; Characteristics of Sulfobacillus acidophilus sp. nov. and other moderately thermophilic mineral-sulphide-oxidizing bacteria. Microbiology141:775–783
    [Google Scholar]
  30. Norris P. R., Burton N. P., Foulis N. A. M.. 2000; Acidophiles in bioreactor mineral processing. Extremophiles4:71–76[CrossRef]
    [Google Scholar]
  31. Okibe N., Johnson D. B.. 2004; Biooxidation of pyrite by defined mixed cultures of moderately thermophilic acidophiles in pH-controlled bioreactors: the significance of microbial interactions. Biotechnol Bioeng87:574–583[CrossRef]
    [Google Scholar]
  32. Okibe N., Gericke M., Hallberg K. B., Johnson D. B.. 2003; Enumeration and characterization of acidophilic microorganisms isolated from a pilot plant stirred tank bioleaching operation. Appl Environ Microbiol69:1936–1943[CrossRef]
    [Google Scholar]
  33. Plumb J. J., Hawkes R. B., Franzman P. D.. 2006; The microbiology of moderately thermophilic and transiently thermophilic ore heaps. In Biomining pp217–235 Edited by Rawlings D. E., Johnson D. B.. Berlin: Springer;
    [Google Scholar]
  34. Rawlings D. E.. 2005; Characteristics and adaptability of iron- and sulfur-oxidizing microorganisms used for the recovery of metals from minerals and their concentrates. Microb Cell Fact4:13http://www.microbialcellfactories.com/content/4/1/13[CrossRef]
    [Google Scholar]
  35. Rawlings D. E., Johnson D. B.. (editors) 2007; Biomining Heidelberg: Springer;
    [Google Scholar]
  36. Rawlings D. E., Silver S.. 1995; Mining with microbes. Bio/Technology13:773–779[CrossRef]
    [Google Scholar]
  37. Rawlings D. E., Dew D., du Plessis C.. 2003; Biomineralization of metal-containing ores and concentrates. Trends Biotechnol21:38–44[CrossRef]
    [Google Scholar]
  38. Rodriguez-Leiva M., Tributsch H.. 1988; Morphology of bacterial leaching patterns by Thiobacillus ferrooxidans on pyrite. Arch Microbiol149:401–405[CrossRef]
    [Google Scholar]
  39. Sand W., Gehrke T.. 2006; Extracellular polymeric substances mediate bioleaching/biocorrosion via interfacial processes involving iron(III) ions and acidophilic bacteria. Res Microbiol157:49–56[CrossRef]
    [Google Scholar]
  40. Sand W., Gehrke T., Hallmann R., Schippers A.. 1995; Sulfur chemistry, biofilm, and the (in)direct attack mechanism – critical evaluation of bacterial leaching. Appl Microbiol Biotechnol43:961–966[CrossRef]
    [Google Scholar]
  41. Stott M. B., Watling H. R., Franzmann P. D., Sutton D. C.. 2000; The role of iron-hydroxy precipitates in the passivation of chalcopyrite during bioleaching. Miner Eng13:1117–1127[CrossRef]
    [Google Scholar]
  42. Temple K. L., Colmer A. R.. 1951; The autotrophic oxidation of iron by a new bacterium: Thiobacillus ferrooxidans . J Bacteriol62:605–611
    [Google Scholar]
  43. Tourova T. P., Poltoraus A. B., Lebedeva I. A., Tsaplina I. A., Bogdanova T. I., Karavaiko G. I.. 1994; 16S ribosomal RNA (rDNA) sequence analysis and phylogenetic position of Sulfobacillus thermosulfidooxidans . Syst Appl Microbiol17:509–512
    [Google Scholar]
  44. Tuffin I. M., Deane S. M., Rawlings D. E., de Groot P.. 2005; An unusual Tn 21 -like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus . Microbiology151:3027–3039[CrossRef]
    [Google Scholar]
  45. Tuffin I. M., Hector S. B., Deane S. M., Rawlings D. E.. 2006; The resistance determinants of a highly arsenic resistant strain of Leptospirillum ferriphilum isolated from a commercial biooxidation tank. Appl Environ Microbiol72:2247–2253[CrossRef]
    [Google Scholar]
  46. van Aswegen P. C., van Niekerk J., Olivier W.. 2007; The BIOX™ process for the treatment of refractory gold concentrates. In Biomining pp1–34 Edited by Rawlings D. E., Johnson D. B.. Heidelberg: Springer;
    [Google Scholar]
  47. Waksman S. A., Joffe J. S.. 1921; Acid production by a new sulfur-oxidizing bacterium. Science53:216[CrossRef]
    [Google Scholar]
  48. Yahya A., Johnson D. B.. 2002; Bioleaching of pyrite at low pH and low redox potentials by novel mesophilic Gram-positive bacteria. Hydrometallurgy63:181–188[CrossRef]
    [Google Scholar]
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