@article{mbs:/content/journal/micro/10.1099/mic.0.2006/001107-0, author = "Feng, Lu and Perepelov, Andrei V. and Zhao, Guang and Shevelev, Sergei D. and Wang, Quan and Senchenkova, Sof'ya N. and Shashkov, Alexander S. and Geng, Yunqi and Reeves, Peter R. and Knirel, Yuriy A. and Wang, Lei", title = "Structural and genetic evidence that the Escherichia coli O148 O antigen is the precursor of the Shigella dysenteriae type 1 O antigen and identification of a glucosyltransferase gene", journal= "Microbiology", year = "2007", volume = "153", number = "1", pages = "139-147", doi = "https://doi.org/10.1099/mic.0.2006/001107-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2006/001107-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "ROESY, rotating Overhauser effect spectroscopy", keywords = "TOCSY, total correlation spectroscopy", keywords = "CAT, chloramphenicol acetyltransferase", keywords = "HMBC, heteronuclear multiple band correlation", keywords = "HSQC, heteronuclear single quantum correlation", keywords = "MLEE, multilocus enzyme electrophoresis", abstract = " Shigella dysenteriae type 1 is the most virulent serotype of Shigella. Enterotoxigenic Escherichia coli O148 is pathogenic and can cause diarrhoea. The following structure was established for the tetrasaccharide repeating unit of the E. coli O148 O antigen: →3)-α-L-Rhap-(1→3)-α-L-Rhap-(1→2)-α-D-Glcp-(1→3)-α-D-GlcpNAc-(1→. This differs from the structure reported earlier for S. dysenteriae type 1 by having a glucose (Glc) residue in place of a galactose (Gal) residue. The two bacteria also have the same genes for O antigen synthesis, with the same organization and high level of DNA identity, except that in S. dysenteriae type 1 wbbG is interrupted by a deletion, and a galactosyltransferase gene wbbP located on a plasmid is responsible for the transfer of galactose to make a novel antigenic epitope of the O antigen. The S. dysenteriae type 1 O antigen was reconstructed by replacing the E. coli O148 wbbG gene with the wbbP gene, and it had the LPS structure and antigenic properties of S. dysenteriae type 1, indicating that the S. dysenteriae type 1 O antigen evolved from that of E. coli O148. It was also confirmed that wbbG of E. coli O148 is a glucosyltransferase gene, and two serotype-specific genes of E. coli O148 and S. dysenteriae type 1 were identified.", }