1887

Abstract

type 1 is the most virulent serotype of . Enterotoxigenic O148 is pathogenic and can cause diarrhoea. The following structure was established for the tetrasaccharide repeating unit of the O148 O antigen: →3)--L-Rha-(1→3)--L-Rha-(1→2)--D-Glc-(1→3)--D-GlcNAc-(1→. This differs from the structure reported earlier for type 1 by having a glucose (Glc) residue in place of a galactose (Gal) residue. The two bacteria also have the same genes for O antigen synthesis, with the same organization and high level of DNA identity, except that in type 1 is interrupted by a deletion, and a galactosyltransferase gene located on a plasmid is responsible for the transfer of galactose to make a novel antigenic epitope of the O antigen. The type 1 O antigen was reconstructed by replacing the O148 gene with the gene, and it had the LPS structure and antigenic properties of type 1, indicating that the type 1 O antigen evolved from that of O148. It was also confirmed that of O148 is a glucosyltransferase gene, and two serotype-specific genes of O148 and type 1 were identified.

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2007-01-01
2019-12-13
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