1887

Abstract

Streptomycetes belong to the ecologically important bacterial population within soil, which is also inhabited by many fungi. The highly chitinolytic and the ascomycete were chosen as models to test for interactions among bacteria and fungi. In medium lacking a soluble carbon source, individually cultivated spores of the bacterium and the fungus do not germinate. However, as shown by viability tests, cultivation of a mixture of both spore types provokes successive events: (i) stimulation of the germination of spores, (ii) initiation of the outgrowth of some fungal spores to which the chitinase ChiO1 adheres, (iii) massive extension of viable networks of hyphae at the expense of fungal hyphae and (iv) balanced proliferation of closely interacting fungal and hyphae. The replacement of the wild-type strain by a chromosomal disruption mutant (ΔC), lacking production of the extracellular chitin-binding protein CHB1 but still secreting the chitinase ChiO1, provokes (v) germination of each spore type, (vi) retarded development of both partners, followed by (vii) preferential proliferation of the fungus. Together with biochemical and immunomicroscopy studies, the data support the conclusion that CHB1 molecules aggregate to an extracellular matrix, maintaining a close contact, followed by several concerted responses of the bacterium and the fungus.

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2007-02-01
2019-10-13
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