1887

Abstract

The activity of glycogen-accumulating organisms (GAOs) in enhanced biological phosphorus removal (EBPR) wastewater treatment plants has been proposed as one cause of deterioration of EBPR. Putative GAOs from the , spp. (including ), were studied in full-scale EBPR plants to determine their distribution, abundance and ecophysiology. Fluorescence hybridization (FISH) demonstrated that spp. were generally low in abundance; however, in one plant surveyed, Cluster 2 constituted 9 % of all . FISH combined with microautoradiography revealed that both Cluster 1 and Cluster 2 were capable of taking up a narrow range of substrates including acetate, propionate, pyruvate and glucose under anaerobic and aerobic conditions. Formate, butyrate, ethanol and several other substrates were not taken up. Cluster 2 demonstrated a phenotype consistent with the current metabolic model for GAOs – anaerobic assimilation of acetate and reduction to polyhydroxyalkanoates (PHA) using the glycolytic pathway, and aerobic consumption of PHA. Polyphosphate-accumulating organisms (PAOs, ‘ Accumulibacter phosphatis’) and other putative GAOs (‘ Competibacter phosphatis’) co-existed in two plants with Cluster 2 , but in both plants, the latter organisms were more abundant. Thus Cluster 2 can be relatively abundant and could be carbon competitors of PAOs and other GAOs in EBPR plants.

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2007-01-01
2020-07-16
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