Gutta percha, the trans-isomer of polyisoprene, is being used for several technical applications due to its resistance to biological degradation. In the past, several attempts to isolate micro-organisms capable of degrading chemically pure poly(trans-1,4-isoprene) have failed. This is the first report on axenic cultures of bacteria capable of degrading gutta percha. From about 100 different habitats and enrichment cultures, six bacterial strains were isolated which utilize synthetic poly(trans-1,4-isoprene) as sole carbon and energy source for growth. All isolates were assigned to the genus Nocardia based on 16S rRNA gene sequences. Four isolates were identified as strains of Nocardia nova (L1b, SH22a, SEI2b and SEII5a), one isolate was identified as a strain of Nocardia jiangxiensis (SM1) and the other as a strain of Nocardia takedensis (WE30). In addition, the type strain of N. takedensis obtained from a culture collection (DSM 44801T) was shown to degrade poly(trans-1,4-isoprene). Degradation of poly(trans-1,4-isoprene) by these seven strains was verified in mineralization experiments by determining the release of CO2. All seven strains were also capable of mineralizing poly(cis-1,4-isoprene) and to use this polyisoprenoid as a carbon and energy source for growth. Mineralization of poly(trans-1,4-isoprene) after 80 days varied from 3 % (strain SM1) to 54 % (strain SEI2b) and from 34 % (strain L1b) to 43 % (strain SH22a) for the cis-isomer after 78 days. In contrast, Gordonia polyisoprenivorans strain VH2, which was previously isolated as a potent poly(cis-1,4-isoprene)-degrading bacterium, was unable to degrade poly(trans-1,4-isoprene). Scanning electron microscopy revealed cavities in solid materials prepared from poly(trans-1,4-isoprene) and also from poly(cis-1,4-isoprene) after incubation with N. takedensis strain WE30 or with N. nova strain L1b, whereas solid poly(trans-1,4-isoprene) material remained unaffected if incubated with G. polyisoprenivorans strain VH2 or under sterile conditions.
ArvanitoyannisI., KolokurisI., NakayamaA.,
AibaS.1998; Preparation and study of novel biodegradable blends based on gelatinized starch and 1,4-transpolyisoprene (gutta percha) for food packaging or biomedical applications. Carbohydr Polym 34:291–302
BraazR., FischerP.,
JendrossekD.2004; Novel type of heme-dependent oxygenase catalyzes oxidative cleavage of rubber (poly- cis -1,4-isoprene. Appl Environ Microbiol 70:7388–7395[CrossRef]
EnokiM., DoiY.,
IwataT.2003; Oxidative degradation of trans -1,4-polyisoprene cast films and single crystals by enzyme-mediator systems. Macromol Biosci 3:668–674[CrossRef]
IbrahimE. M. A., LuftmannH., ArenskötterM.,
SteinbüchelA.2006; Identification of poly( cis -1,4-isoprene) degradation intermediates during growth of moderately thermophilic Actinomycetes on rubber and cloning of a functional lcp homologue from Nocardia farcinica strain E1. Appl Environ Microbiol 72:3375–3382[CrossRef]
JendrossekD., TomassiG.,
KroppenstedtR.1997; Bacterial degradation of natural rubber: a privilege of actinomycetes?. FEMS Microbiol Lett 150:179–188[CrossRef]
KupletskayaM. B., KuznetsovaV. M.,
ZhukovaS. V.1960; Microbiological maceration of Eucommia leaves. III. Disintegration of gutta and resins in the process of fermentation of the leaves. Mikrobiologiia 29:259–265 in Russian
LinosA., SteinbüchelA.1998; Microbial degradation of natural and synthetic rubbers by novel bacteria belonging to the genus Gordona . Kautsch Gummi Kunstst 51:496–499
LinosA., BerekaaM. M., ReicheltR., KellerU., SchmittJ., FlemmingH. C., KroppenstedtR. M., SteinbüchelA.2000; Biodegradation of cis -1,4-polyisoprene rubbers by distinct actinomycetes: microbial strategies and detailed surface analysis. Appl Environ Microbiol 66:1639–1645[CrossRef]
RaineyF. A., Ward-RaineyN., KroppenstedtR. M.,
StackebrandtE.1996; The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46:1088–1092[CrossRef]
RoseK., SteinbüchelA.2005; Biodegradation of natural rubber and related compounds: recent insights into a hardly understood catabolic capability of microorganisms. Appl Environ Microbiol 71:2803–2812[CrossRef]
RoseK., TenbergeK. B., SteinbüchelA.2005; Identification and characterization of genes from Streptomyces sp. strain K30 responsible for clear zone formation on natural rubber latex and poly( cis -1,4-isoprene) rubber degradation. Biomacromolecules 6:180–188[CrossRef]
ThompsonJ. D., GibsonT. J., PlewniakF., JeanmouginF.,
HigginsD. G.1997; The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882[CrossRef]