1887

Abstract

Marine sponges harbour abundant and diverse bacterial communities, providing an ideal environment for bacterial cell-density-dependent cell–cell signalling, termed quorum sensing. The marine sponge symbiont sp. KLH11 produces mainly long chain acylhomoserine lactones (AHLs) and has been developed as a quorum sensing model for roseobacterial sponge symbionts. Two pairs of homologues were identified by genetic screening and were designated and (sponge-associated symbiont locus A or B, homologue). In this study, we identified a third -type gene, named . The gene does not have a cognate homologue present at an adjacent locus and thus is an AHL synthase solo. The gene is required for production of long-chain hydroxylated AHLs, contributes to AHL pools and modestly influences flagellar motility in KLH11. A triple mutant for all -type genes cannot produce AHLs, but still synthesizes -coumaroyl-homoserine lactone.

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2015-01-01
2019-11-21
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