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Volume 161, Issue 1

A solo -type gene directs acylhomoserine lactone synthesis and contributes to motility control in the marine sponge symbiont sp. KLH11 Free

Abstract

Marine sponges harbour abundant and diverse bacterial communities, providing an ideal environment for bacterial cell-density-dependent cell–cell signalling, termed quorum sensing. The marine sponge symbiont sp. KLH11 produces mainly long chain acylhomoserine lactones (AHLs) and has been developed as a quorum sensing model for roseobacterial sponge symbionts. Two pairs of homologues were identified by genetic screening and were designated and (sponge-associated symbiont locus A or B, homologue). In this study, we identified a third -type gene, named . The gene does not have a cognate homologue present at an adjacent locus and thus is an AHL synthase solo. The gene is required for production of long-chain hydroxylated AHLs, contributes to AHL pools and modestly influences flagellar motility in KLH11. A triple mutant for all -type genes cannot produce AHLs, but still synthesizes -coumaroyl-homoserine lactone.

  • Received:
  • Accepted:
  • Published Online:
© 2015 The Authors
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2015-01-01
2024-04-24
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A solo luxI-type gene directs acylhomoserine lactone synthesis and contributes to motility control in the marine sponge symbiont Ruegeria sp. KLH11
Microbiology 161, 50 (2015); https://doi.org/10.1099/mic.0.083956-0
/content/journal/micro/10.1099/mic.0.083956-0
/content/journal/micro/10.1099/mic.0.083956-0
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