1887

Abstract

is responsible for swine pleuropneumonia, a respiratory disease that causes significant global economic loss. Its virulence depends on many factors, such as capsular polysaccharides, RTX toxins and iron-acquisition systems. Analysis of virulence may require easy-to-use models that approximate mammalian infection and avoid ethical issues. Here, we investigate the potential use of the wax moth as an informative model for infection. Genotypically distinct clinical isolates were able to kill larvae at 37 °C but had different LD values, ranging from 10 to 10 c.f.u. per larva. The most virulent isolate (1022) was able to persist and replicate within the insect, while the least virulent (780) was rapidly cleared. We observed a decrease in haemocyte concentration, aggregation and DNA damage post-infection with isolate 1022. Melanization points around bacterial cells were observed in the fat body and pericardial tissues of infected , indicating vigorous cell and humoral immune responses close to the larval dorsal vessel. As found in pigs, an . mutant was significantly attenuated for infection in the model. Additionally, the model could be used to assess the effectiveness of several antimicrobial agents against . is a suitable inexpensive alternative infection model that can be used to study the virulence of , as well as assess the effectiveness of antimicrobial agents against this pathogen.

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2015-02-01
2019-10-14
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