1887

Abstract

NusG, a well-conserved protein in all the three forms of life, is involved in transcription elongation and termination, as well as in the process of transcription–translation coupling. The existence of species-specific functional, as well as conformational, divergences in NusG makes it an attractive transcription factor to study, especially if it originates from a pathogen. Here, we report functional and conformational characterizations of the (Mtb) protein Rv0639 that has been annotated as a homologue of NusG. Rv0639 failed to complement the functions of NusG (Ec NusG) and did not exhibit any signature of a transcription elongation–termination factor. However, it retained the ability to bind to its cognate ribosomal protein S10 (Rv0700). Compared with Ec NusG, Rv0639 possesses unique conformational features characterized by altered secondary structures in the C-terminal domain (CTD), an unusually long and disordered linker region between the N-terminal domain (NTD) and CTD, and a folding of its NTD over its CTD. This unusual folded conformation could have imparted specialized functions to this protein, required to adapt the physiology of Mtb We speculate that in the absence of a bona fide RfaH, a NusG paralogue that is involved in pathogenicity in , Rv0639 functions as an RfaH-like factor and is involved in pathogenicity using unidentified -like sequences in the Mtb genome. And hence, we reannotate Rv0639 as a paralogue of NusG, instead of a homologue.

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2015-01-01
2019-11-14
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