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Volume 161, Issue 2

Absence of YbeY RNase compromises the growth and enhances the virulence plasmid gene expression of O:3 Free

Abstract

YbeY was recently recognized as an endoribonuclease playing a role in ribosome biosynthesis. In it functions as a single-strand-specific RNase that processes the 3′ end of the 16S rRNA and is crucial for the late-stage 70S ribosome quality control system. Here we report that YbeY is not essential in serotype O:3, yet its absence strongly compromised the bacterium. The lack of YbeY resulted in misprocessing of 16S rRNA and a severe decrease of growth rate with complete growth arrest observed at elevated temperatures. Moreover, a mutation severely disturbed regulation of the virulence plasmid (pYV) genes and affected the expression of regulatory small RNA species. Transcription of the pYV genes was upregulated in the mutant at 22 °C; the same genes were repressed in the wild-type bacterium. Furthermore, inactivation impaired many virulence-related features, such as resistance to elevated temperature and acid, and hindered utilization of different carbohydrates. In addition, the mutant strain showed decreased infectivity in a tissue culture infection model, especially at the stage of cell adhesion. Taken together, this study demonstrates the crucial role of YbeY in O:3 physiology and pathogenicity.

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© 2015 The Authors
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2015-02-01
2024-04-25
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Absence of YbeY RNase compromises the growth and enhances the virulence plasmid gene expression of Yersinia enterocolitica O:3
Microbiology 161, 285 (2015); https://doi.org/10.1099/mic.0.083097-0
/content/journal/micro/10.1099/mic.0.083097-0
/content/journal/micro/10.1099/mic.0.083097-0
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