1887

Abstract

The type II protein secretion (T2S) system of secretes over 25 proteins, including novel proteins that have no similarity to proteins of known function. T2S is also critical for the ability of to grow within its natural amoebal hosts, including , and . Thus, T2S has an important role in the natural history of legionnaires’ disease. Our previous work demonstrated that the novel T2S substrate NttA promotes intracellular infection of , whereas the secreted RNase SrnA, acyltransferase PlaC, and metalloprotease ProA all promote infection of and . In this study, we determined that another novel T2S substrate that is specific to , designated NttC, is unique in being required for intracellular infection of but not for infection of or . Expanding our repertoire of amoebal hosts, we determined that is susceptible to infection by strains 130b, Philadelphia-1 and Paris. Furthermore, T2S and, more specifically, NttA, NttC and PlaC were required for infection of . Taken together, these data demonstrate that the T2S system of is critical for infection of at least four types of aquatic amoebae and that the importance of the individual T2S substrates varies in a host cell-specific fashion. Finally, it is now clear that novel T2S-dependent proteins that are specific to the genus are particularly important for infection of key, environmental hosts.

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2014-12-01
2019-09-19
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