1887

Abstract

Hydrogen peroxide (HO) is produced by several members of the genus mainly through the pyruvate oxidase SpxB under aerobic growth conditions. The acute toxic nature of HO raises the interesting question of how streptococci cope with intrinsically produced HO, which subsequently accumulates in the microenvironment and threatens the closely surrounding population. Here, we investigate the HO susceptibility of oral and and elucidate potential mechanisms of how they protect themselves from the deleterious effect of HO. Both organisms are considered primary colonizers and occupy the same intraoral niche making them potential targets for HO produced by other species. We demonstrate that produces relatively more HO and has a greater ability for resistance to HO stress. Functional studies show that, unlike in , HO resistance is not dependent on a functional SpxB and confirms the important role of the ferritin-like DNA-binding protein Dps. However, the observed increased HO resistance of over is likely to be caused by an oxidative stress protection machinery present even under anaerobic conditions, while requires a longer period of time for adaptation. The ability to produce more HO and be more resistant to HO might aid in the competitive oral biofilm environment, since it is lower in abundance yet manages to survive quite efficiently in the oral biofilm.

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2014-12-01
2020-09-27
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