1887

Abstract

can acquire a higher tolerance to tetracycline by increasing the gene dosage of its resistance gene . In this study, we estimated the multiplication effect of on tetracycline tolerance. Cells harbouring multiple copies of were found to comprise approximately 30 % of the total tetracycline-resistant cell population when selected on medium containing 10 µg tetracycline ml. Disruption of resulted in a significant decrease in the frequency of amplification. Although four direct repeats exist around , the majority of amplicons were found to be flanked by non-homologous sequences, indicating that the initial duplication of can occur largely through RecA-independent recombination. The correlation between the copy number and the MIC values for tetracycline indicated that more than three copies of were required for tolerance to 10 µg tetracycline ml. Thus, the RecA-dependent expansion step appears to be necessary for developing significant tetracycline tolerance mediated by amplification.

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2014-11-01
2019-11-19
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