1887

Abstract

Osmoregulated periplasmic glucans (OPGs) are general constituents of many proteobacteria. OPGs are important factors required for full virulence in many pathogens including . causes the soft-rot disease in a wide range of plant species. The pleiotropic phenotype of -negative strains includes total loss of virulence and motility, and is linked to the constitutive activation of the RcsCDB phosphorelay, deduced from expression analysis of genes of the RcsCDB regulon. The constitutive activation of the RcsCDB phosphorelay in an -negative strain was demonstrated by direct analysis of the phosphorylation level of the RcsB regulator protein by using a Phos-tag retardation gel approach, and was correlated with the phenotype and the expression of motility genes. Data revealed a low level of RcsB phosphorylated form in the wild-type strain and a slight increase of phosphorylation in mutant strains sufficient to induce the pleiotropic phenotype observed.

Funding
This study was supported by the:
  • Centre National de la Recherche Scientifique
  • Ministère de l’Education Nationale et de la Recherche
  • Université des Sciences et Technologies
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2014-12-01
2024-04-25
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References

  1. Arellano-Reynoso B., Lapaque N., Salcedo S., Briones G., Ciocchini A. E., Ugalde R., Moreno E., Moriyón I., Gorvel J. P. ( 2005). Cyclic beta-1,2-glucan is a Brucella virulence factor required for intracellular survival. Nat Immunol 6:618–625 [View Article][PubMed]
    [Google Scholar]
  2. Barbieri C. M., Stock A. M. ( 2008). Universally applicable methods for monitoring response regulator aspartate phosphorylation both in vitro and in vivo using Phos-tag-based reagents. Anal Biochem 376:73–82 [View Article][PubMed]
    [Google Scholar]
  3. Bertani G. ( 2004). Lysogeny at mid-twentieth century: P1, P2, and other experimental systems. J Bacteriol 186:595–600 [View Article][PubMed]
    [Google Scholar]
  4. Bohin J.-P., Lacroix J. M. ( 2006). Osmoregulation in the periplasm. The Periplasm325–341 Ehrmann Washington M. DC: American Society for Microbiology;
    [Google Scholar]
  5. Bontemps-Gallo S., Madec E., Dondeyne J., Delrue B., Robbe-Masselot C., Vidal O., Prouvost A. F., Boussemart G., Bohin J. P., Lacroix J. M. ( 2013). Concentration of osmoregulated periplasmic glucans (OPGs) modulates the activation level of the RcsCD RcsB phosphorelay in the phytopathogen bacteria Dickeya dadantii.. Environ Microbiol 15:881–894 [View Article][PubMed]
    [Google Scholar]
  6. Bontemps-Gallo S., Madec E., Lacroix J. M. ( 2014). Inactivation of pecS restores the virulence of mutants devoid of osmoregulated periplasmic glucans in the phytopathogenic bacterium Dickeya dadantii.. Microbiology 160:766–777 [View Article][PubMed]
    [Google Scholar]
  7. Bouchart F., Delangle A., Lemoine J., Bohin J. P., Lacroix J. M. ( 2007). Proteomic analysis of a non-virulent mutant of the phytopathogenic bacterium Erwinia chrysanthemi deficient in osmoregulated periplasmic glucans: change in protein expression is not restricted to the envelope, but affects general metabolism. Microbiology 153:760–767 [View Article][PubMed]
    [Google Scholar]
  8. Bouchart F., Boussemart G., Prouvost A. F., Cogez V., Madec E., Vidal O., Delrue B., Bohin J. P., Lacroix J. M. ( 2010). The virulence of a Dickeya dadantii 3937 mutant devoid of osmoregulated periplasmic glucans is restored by inactivation of the RcsCD-RcsB phosphorelay. J Bacteriol 192:3484–3490 [View Article][PubMed]
    [Google Scholar]
  9. Bradford M. M. ( 1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 [View Article][PubMed]
    [Google Scholar]
  10. Cano D. A., Domínguez-Bernal G., Tierrez A., Garcia-Del Portillo F., Casadesús J. ( 2002). Regulation of capsule synthesis and cell motility in Salmonella enterica by the essential gene igaA.. Genetics 162:1513–1523[PubMed]
    [Google Scholar]
  11. Clarke D. J. ( 2010). The Rcs phosphorelay: more than just a two-component pathway. Future Microbiol 5:1173–1184 [View Article][PubMed]
    [Google Scholar]
  12. Hommais F., Oger-Desfeux C., Van Gijsegem F., Castang S., Ligori S., Expert D., Nasser W., Reverchon S. ( 2008). PecS is a global regulator of the symptomatic phase in the phytopathogenic bacterium Erwinia chrysanthemi 3937. J Bacteriol 190:7508–7522 [View Article][PubMed]
    [Google Scholar]
  13. Laemmli U. K. ( 1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685 [View Article][PubMed]
    [Google Scholar]
  14. Lukat G. S., Stock J. B. ( 1993). Response regulation in bacterial chemotaxis. J Cell Biochem 51:41–46 [View Article][PubMed]
    [Google Scholar]
  15. Mahajan-Miklos S., Tan M. W., Rahme L. G., Ausubel F. M. ( 1999). Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model. Cell 96:47–56 [View Article][PubMed]
    [Google Scholar]
  16. Majdalani N., Gottesman S. ( 2005). The Rcs phosphorelay: a complex signal transduction system. Annu Rev Microbiol 59:379–405 [View Article][PubMed]
    [Google Scholar]
  17. Page F., Altabe S., Hugouvieux-Cotte-Pattat N., Lacroix J. M., Robert-Baudouy J., Bohin J. P. ( 2001). Osmoregulated periplasmic glucan synthesis is required for Erwinia chrysanthemi pathogenicity. J Bacteriol 183:3134–3141 [View Article][PubMed]
    [Google Scholar]
  18. Perombelon M. C. M. ( 2002). Potato diseases caused by soft-rot erwinias: an overview of pathogenesis. Plant Pathol 51:1–12 [View Article]
    [Google Scholar]
  19. Sheridan R. C., McCullough J. F., Wakefield Z. T., Allcock H. R., Walsh E. J. ( 1972). Phosphoramidic acid and its salts. Inorg Synth 13:23–26
    [Google Scholar]
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