1887

Abstract

Sro7 and Sro77 are homologues of the tumour suppressor lethal giant larvae (Lgl), which regulates cell polarity in epithelial cells. Here, we showed that double mutation of / was defective in colony growth. The colony of the / double deletion was much smaller than the WT and appeared to be round with a smooth surface, compared with the WT. Analysis using transmission electron microscopy revealed multiple defects of the colony cells, including multiple budding, multiple nuclei, cell lysis and dead cells, suggesting that the double deletion caused defects in cell polarity and cell wall integrity (CWI). Overexpression of , one of the central regulators of cell polarity and CWI, fully recovered the Δ/Δ phenotype. We further demonstrated that Δ/Δ caused a decrease of the GTP-bound, active Rho1, which in turn caused an upregulation of . Deletion of in Δ/Δ (Δ/Δ/Δ) recovered the cell growth and colony morphology, similar to WT. Our results suggested that the tumour suppressor homologue / regulated cell proliferation and yeast colony development via the Rho1–Tor1 pathway.

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2014-10-01
2019-11-18
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