1887

Abstract

, a Gram-negative anaerobic bacterium, is an important pathogen in periodontal disease. This bacterium possesses genes encoding all known components of the type IX secretion system (T9SS). mutants deficient in genes orthologous to the T9SS-encoding genes , and were constructed. All , and single mutants lacked the surface layer (S-layer) and expressed less-glycosylated versions of the S-layer glycoproteins TfsA and TfsB. In addition, these mutants exhibited decreased haemagglutination and increased biofilm formation. Comparison of the proteins secreted by the and WT strains revealed that the secretion of several proteins containing C-terminal domain (CTD)-like sequences is dependent on the gene. These results indicate that the T9SS is functional in and contributes to the translocation of CTD proteins to the cell surface or into the extracellular milieu.

Funding
This study was supported by the:
  • Grants-in-Aid for Scientific Research
  • Global COE Program at Nagasaki University
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2014-10-01
2021-10-25
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