The Neurospora crassa genome encodes two 1,3-α-glucan synthases. One of these 1,3-α-glucan synthase genes, ags-1, was shown to be required for the synthesis of 1,3-α-glucan in the aerial hyphae and macroconidia cell walls. 1,3-α-Glucan was found in the conidia cell wall, but was absent from the vegetative hyphae cell wall. Deletion of ags-1 affected conidial development. Δags-1 produced only 5 % as many conidia as the WT and most of the conidia produced by Δags-1 were not viable. The ags-1 upstream regulatory elements were shown to direct cell-type-specific expression of red fluorescent protein in conidia and aerial hyphae. A haemagglutinin-tagged AGS-1 was found to be expressed in aerial hyphae and conidia. The research showed that 1,3-α-glucan is an aerial hyphae and conidia cell wall component, and is required for normal conidial differentiation.
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Funding
This study was supported by the:
National Institutes of Health
(Award R01 GM078589)
National Science Foundation
(Award DBI0923133)
University at Buffalo Foundation
National Institutes of Health
(Award P01 GM068087)
BatesS.,
HughesH. B.,
MunroC. A.,
ThomasW. P.,
MacCallumD. M.,
BertramG.,
AtrihA.,
FergusonM. A.,
BrownA. J.& other authors (2006). Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans. J Biol Chem 281:90–98 [View Article][PubMed]
BeauvaisA.,
BozzaS.,
KniemeyerO.,
FormosaC.,
BalloyV.,
HenryC.,
RobersonR. W.,
DagueE.,
ChignardM.& other authors (2013). Deletion of the α-(1,3)-glucan synthase genes induces a restructuring of the conidial cell wall responsible for the avirulence of Aspergillus fumigatus. PLoS Pathog 9:e1003716 [View Article][PubMed]
BorkovichK. A.,
AlexL. A.,
YardenO.,
FreitagM.,
TurnerG. E.,
ReadN. D.,
SeilerS.,
Bell-PedersenD.,
PaiettaJ.& other authors (2004). Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. Microbiol Mol Biol Rev 68:1–108 [View Article][PubMed]
BowmanB. J.,
DraskovicM.,
FreitagM.,
BowmanE. J.(2009). Structure and distribution of organelles and cellular location of calcium transporters in Neurospora crassa. Eukaryot Cell 8:1845–1855 [View Article][PubMed]
ColotH. V.,
ParkG.,
TurnerG. E.,
RingelbergC.,
CrewC. M.,
LitvinkovaL.,
WeissR. L.,
BorkovichK. A.,
DunlapJ. C.(2006). A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors. Proc Natl Acad Sci U S A 103:10352–10357 [View Article][PubMed]
GalaganJ. E.,
CalvoS. E.,
BorkovichK. A.,
SelkerE. U.,
ReadN. D.,
JaffeD.,
FitzHughW.,
MaL. J.,
SmirnovS.& other authors (2003). The genome sequence of the filamentous fungus Neurospora crassa. Nature 422:859–868 [View Article][PubMed]
HochstenbachF.,
KlisF. M.,
van den EndeH.,
van DonselaarE.,
PetersP. J.,
KlausnerR. D.(1998). Identification of a putative alpha-glucan synthase essential for cell wall construction and morphogenesis in fission yeast. Proc Natl Acad Sci U S A 95:9161–9166 [View Article][PubMed]
LatgéJ. P.,
MouynaI.,
TekaiaF.,
BeauvaisA.,
DebeaupuisJ. P.,
NiermanW.(2005). Specific molecular features in the organization and biosynthesis of the cell wall of Aspergillus fumigatus. Med Mycol 43:Suppl 1S15–S22 [View Article][PubMed]
LindingR.,
RussellR. B.,
NeduvaV.,
GibsonT. J.(2003). GlobPlot: exploring protein sequences for globularity and disorder. Nucleic Acids Res 31:3701–3708 [View Article][PubMed]
MaddiA.,
FreeS. J.(2010). α-1,6-Mannosylation of N-linked oligosaccharide present on cell wall proteins is required for their incorporation into the cell wall in the filamentous fungus Neurospora crassa. Eukaryot Cell 9:1766–1775 [View Article][PubMed]
MargolinB. S.,
FrietagM.,
SelkerE. U.(1997). Improved plasmids for gene targeting at the his-3 locus of Neurospora crassa.. Fungal Genet Newslett 44:34–36
PardiniG.,
De GrootP. W.,
CosteA. T.,
KarababaM.,
KlisF. M.,
de KosterC. G.,
SanglardD.(2006). The CRH family coding for cell wall glycosylphosphatidylinositol proteins with a predicted transglycosidase domain affects cell wall organization and virulence of Candida albicans. J Biol Chem 281:40399–40411 [View Article][PubMed]
RappleyeC. A.,
EngleJ. T.,
GoldmanW. E.(2004). RNA interference in Histoplasma capsulatum demonstrates a role for alpha-(1,3)-glucan in virulence. Mol Microbiol 53:153–165 [View Article][PubMed]
RappleyeC. A.,
EissenbergL. G.,
GoldmanW. E.(2007).Histoplasma capsulatum alpha-(1,3)-glucan blocks innate immune recognition by the beta-glucan receptor. Proc Natl Acad Sci U S A 104:1366–1370 [View Article][PubMed]
Ruiz-HerreraJ.,
ElorzaM. V.,
ValentínE.,
SentandreuR.(2006). Molecular organization of the cell wall of Candida albicans and its relation to pathogenicity. FEMS Yeast Res 6:14–29 [View Article][PubMed]
SesteroC. M.,
ScalaroneG. M.(2007). Detection of the surface antigens BAD1 and alpha-(1,3)-glucan in six different strains of Blastomyces dermatitidus using monoclonal antibodies. J Med Biol Sci 1:1–7
SpringerM. L.,
YanofskyC.(1989). A morphological and genetic analysis of conidiophore development in Neurospora crassa. Genes Dev 3:559–571 [View Article][PubMed]
VosA.,
DekkerN.,
DistelB.,
LeunissenJ. A.,
HochstenbachF.(2007). Role of the synthase domain of Ags1p in cell wall alpha-glucan biosynthesis in fission yeast. J Biol Chem 282:18969–18979 [View Article][PubMed]
YoshimiA.,
SanoM.,
InabaA.,
KokubunY.,
FujiokaT.,
MizutaniO.,
HagiwaraD.,
FujikawaT.,
NishimuraM.& other authors (2013). Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungus. PLoS ONE 8:e54893 [View Article][PubMed]