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Volume 160, Issue 8

1,3-α-glucan synthase, AGS-1, is required for cell wall biosynthesis during macroconidia development Free

Abstract

The genome encodes two 1,3-α-glucan synthases. One of these 1,3-α-glucan synthase genes, , was shown to be required for the synthesis of 1,3-α-glucan in the aerial hyphae and macroconidia cell walls. 1,3-α-Glucan was found in the conidia cell wall, but was absent from the vegetative hyphae cell wall. Deletion of affected conidial development. Δ produced only 5 % as many conidia as the WT and most of the conidia produced by Δ were not viable. The upstream regulatory elements were shown to direct cell-type-specific expression of red fluorescent protein in conidia and aerial hyphae. A haemagglutinin-tagged AGS-1 was found to be expressed in aerial hyphae and conidia. The research showed that 1,3-α-glucan is an aerial hyphae and conidia cell wall component, and is required for normal conidial differentiation.

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • National Institutes of Health (Award R01 GM078589)
  • National Science Foundation (Award DBI0923133)
  • University at Buffalo Foundation
  • National Institutes of Health (Award P01 GM068087)
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2014-08-01
2025-04-27
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/content/journal/micro/10.1099/mic.0.080002-0
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Neurospora crassa 1,3-α-glucan synthase, AGS-1, is required for cell wall biosynthesis during macroconidia development
Microbiology 160, 1618 (2014); https://doi.org/10.1099/mic.0.080002-0
/content/journal/micro/10.1099/mic.0.080002-0
/content/journal/micro/10.1099/mic.0.080002-0
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