@article{mbs:/content/journal/micro/10.1099/mic.0.078840-0, author = "Troskie, Anscha M. and de Beer, Abré and Vosloo, Johan A. and Jacobs, Karin and Rautenbach, Marina", title = "Inhibition of agronomically relevant fungal phytopathogens by tyrocidines, cyclic antimicrobial peptides isolated from Bacillus aneurinolyticus", journal= "Microbiology", year = "2014", volume = "160", number = "9", pages = "2089-2101", doi = "https://doi.org/10.1099/mic.0.078840-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.078840-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "The tyrocidines, a complex of analogous cyclic decapeptides produced by Bacillus aneurinolyticus, exhibited noteworthy activity against a range of phytopathogenic fungi, including Fusarium verticillioides, Fusarium solani and Botrytis cinerea. The activity of the tyrocidine peptide complex (Trc mixture) and purified tyrocidines exhibited minimum inhibition concentrations below 13 µg ml−1 (~10 µM) and was significantly more potent than that of the commercial imidazole fungicide, bifonazole. Although the tyrocidines’ activity was negatively influenced by the presence of Ca2+, it remained unaffected by the presence of Mg2+, Na+ and K+. Microscopic analysis revealed significant impact on the morphology of F. solani and Bot. cinerea including retarded germination and hyperbranching of hyphae. Studies with membrane-impermeable dyes, SYTOX green and propidium iodide suggested that the main mode of action of tyrocidines involves the disruption of fungal membrane integrity. Because of the tyrocidines’ broad spectrum and potent antifungal activity, possible multiple targets reducing the risk of overt resistance and general salt tolerance, they are promising candidates that warrant further investigation as bio-fungicides.", }