RT Journal Article SR Electronic(1) A1 Wübbeler, Jan Hendrik A1 Hiessl, Sebastian A1 Schuldes, Jörg A1 Thürmer, Andrea A1 Daniel, Rolf A1 Steinbüchel, AlexanderYR 2014 T1 Unravelling the complete genome sequence of Advenella mimigardefordensis strain DPN7T and novel insights in the catabolism of the xenobiotic polythioester precursor 3,3′-dithiodipropionate JF Microbiology, VO 160 IS 7 SP 1401 OP 1416 DO https://doi.org/10.1099/mic.0.078279-0 PB Microbiology Society, SN 1465-2080, AB Advenella mimigardefordensis strain DPN7T is a remarkable betaproteobacterium because of its extraordinary ability to use the synthetic disulfide 3,3′-dithiodipropionic acid (DTDP) as the sole carbon source and electron donor for aerobic growth. One application of DTDP is as a precursor substrate for biotechnically synthesized polythioesters (PTEs), which are interesting non-degradable biopolymers applicable for plastics materials. Metabolic engineering for optimization of PTE production requires an understanding of DTDP conversion. The genome of A. mimigardefordensis strain DPN7T was sequenced and annotated. The circular chromosome was found to be composed of 4 740 516 bp and 4112 predicted ORFs, whereas the circular plasmid consisted of 23 610 bp and 24 predicted ORFs. The genes participating in DTDP catabolism had been characterized in detail previously, but knowing the complete genome sequence and with support of Tn5 : : mob-induced mutants, putatively involved transporter proteins and a transcriptional regulator were also identified. Most probably, DTDP is transported into the cell by a specific tripartite tricarboxylate transport system and is then cleaved by the disulfide reductase LpdA, sulfoxygenated by the 3-mercaptopropionate dioxygenase Mdo, activated by the CoA ligase SucCD and desulfinated by the acyl-CoA dehydrogenase-like desulfinase AcdA. Regulation of this pathway is presumably performed by a transcriptional regulator of the xenobiotic response element family. The excessive sulfate that is inevitably produced is secreted by the cells by a unique sulfate exporter of the CPA (cation : proton antiporter) superfamily., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.078279-0