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Volume 160, Issue 5

Analysis of the small RNA in the plant pathogen pv. strain DC3000 Free

Abstract

Bacteria contain small non-coding RNAs (ncRNAs) that are typically responsible for altering transcription, translation or mRNA stability. ncRNAs are important because they often regulate virulence factors and susceptibility to various stresses. Here, the regulation of a recently described ncRNA of DC3000, (now referred to as ), was investigated. A putative RpoE binding site was identified upstream of in strain DC3000. RpoE is shown to regulate the expression of . Also, deletion of results in increased sensitivity to hydrogen peroxide compared with the wild-type strain, suggesting that plays a role in susceptibility to oxidative stress. Furthermore, expression of is shown to be influenced by , suggesting that this ncRNA plays a role in alginate biosynthesis. Structural and comparative genomic analyses show this ncRNA is well conserved among the pseudomonads. The findings provide new information on the regulation and role of this ncRNA in .

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • Fredrick N. Gabler ’93 Memorial Research Endowment
© Microbiology Society
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2014-05-01
2024-04-18
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Analysis of the small RNA spf in the plant pathogen Pseudomonas syringae pv. tomato strain DC3000
Microbiology 160, 941 (2014); https://doi.org/10.1099/mic.0.076497-0
/content/journal/micro/10.1099/mic.0.076497-0
/content/journal/micro/10.1099/mic.0.076497-0
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