1887

Abstract

encounters an array of sugar moieties within the oral cavity due to a varied human diet. One such sugar is β--glucose 1-phosphate (βDG1P), which must be converted to glucose 6-phosphate (G6P) before further metabolism to lactic acid. The conversion of βDG1P to G6P is mediated by β-phosphoglucomutase, which has not been previously observed in any oral streptococci, but has been extensively characterized and the gene designated in . An orthologue was identified in , SMU.1747c, and deletion of the gene resulted in the inability of the deletion strain to convert βDG1P to G6P, indicating that SMU.1747c is a β-phosphoglucomutase and should be designated . In this study, we sought to characterize how deletion of affected known virulence factors of , specifically acid tolerance. The Δ strain showed a decreased ability to survive acid challenge. Additionally, the strain lacking β-phosphoglucomutase had a diminished glycolytic profile compared with the parental strain. Deletion of had a negative impact on the virulence of in the (greater wax worm) animal model. Our results indicate that plays a role at the juncture of carbohydrate metabolism and virulence.

Funding
This study was supported by the:
  • Training Program in Oral Sciences (Award NIH/NIDCR DE- 17157, NIH/NIDCR DE-17425, NIH/NIDCR DE-13683, T90-DE021985 and NIH/NIDCR T32 DE-07165)
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2014-04-01
2021-07-27
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