1887

Abstract

While there is evidence for the persistence of in soil, there are no reports for the other complex (MTC) mycobacteria. Here, soil was inoculated with 10 c.f.u. g , and and subcultured monthly for 12 months. The pathogenicity of mycobacterial colonies, identified by using matrix-assisted laser desorption/ionization time of flight mass spectrometry, was assessed in a mouse model. Moreover, mice were fed with food that contained 16.7 % -contaminated soil. The three tested MTC species survived in soil for 12 months with a final inoculum of 2×10 c.f.u. g for , 150 c.f.u. g for and 2×10 c.f.u. g for . In an experiment that included negative controls, all (5/5) mice inoculated with such and developed 0.03–0.3 granulomas mm in their lungs and spleen and grew mycobacteria; five mice that were inoculated with from soil did not develop granulomas but grew mycobacteria. Furthermore, 0.2–0.4 granulomas mm were observed in the lungs and spleen of 3/5 mice fed with -contaminated soil in the presence of two negative control mice. grew in the stomach, intestine, spleen and lung in 5/5 challenged mice, whereas the negative controls remained -free ( = 0.008, Fisher exact test). This study provides clear evidence that MTC mycobacteria survive in soil, and that remains virulent while in the soil, outside its hosts, for extended periods of time.

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2014-03-01
2021-10-15
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References

  1. Alexander K. A., Laver P. N., Michel A. L., Williams M., van Helden P. D., Warren R. M., Gey van Pittius N. C. ( 2010). Novel Mycobacterium tuberculosis complex pathogen, M. mungi. Emerg Infect Dis 16:1296–1299 [View Article][PubMed]
    [Google Scholar]
  2. Courtenay O., Reilly L. A., Sweeney F. P., Hibberd V., Bryan S., Ul-Hassan A., Newman C., Macdonald D. W., Delahay R. J. & other authors ( 2006). Is Mycobacterium bovis in the environment important for the persistence of bovine tuberculosis. Biol Lett 2:460–462 [View Article][PubMed]
    [Google Scholar]
  3. de Jong B. C., Antonio M., Gagneux S. ( 2010). Mycobacterium africanum – review of an important cause of human tuberculosis in West Africa. PLoS Negl Trop Dis 4:e744 [View Article][PubMed]
    [Google Scholar]
  4. Djelouadji Z., Raoult D., Drancourt M. ( 2011). Palaeogenomics of Mycobacterium tuberculosis: epidemic bursts with a degrading genome. Lancet Infect Dis 11:641–650 [View Article][PubMed]
    [Google Scholar]
  5. Duffield B. J., Young D. A. ( 1985). Survival of Mycobacterium bovis in defined environmental conditions. Vet Microbiol 10:193–197 [View Article][PubMed]
    [Google Scholar]
  6. El Khéchine A., Couderc C., Flaudrops C., Raoult D., Drancourt M. ( 2011). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry identification of mycobacteria in routine clinical practice. PLoS ONE 6:e24720 [View Article][PubMed]
    [Google Scholar]
  7. Fine A. E., Bolin C. A., Gardiner J. C., Kaneene J. B. ( 2011). A study of the persistence of Mycobacterium bovis in the environment under natural weather conditions in Michigan USA. Vet Med Int 2011:765430 [View Article][PubMed]
    [Google Scholar]
  8. Frankel A., Penrose C., Emer J. ( 2009). Cutaneous tuberculosis: a practical case report and review for the dermatologist. J Clin Aesthet Dermatol 2:19–27[PubMed]
    [Google Scholar]
  9. Gagneux S., DeRiemer K., Van T., Kato-Maeda M., de Jong B. C., Narayanan S., Nicol M., Niemann S., Kremer K. & other authors ( 2006). Variable host–pathogen compatibility in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A 103:2869–2873 [View Article][PubMed]
    [Google Scholar]
  10. Ghodbane R., Drancourt M. ( 2013). Non-human sources of Mycobacterium tuberculosis. Tuberculosis (Edinb) 93:589–595 [View Article][PubMed]
    [Google Scholar]
  11. Koeck J. L., Fabre M., Simon F., Daffé M., Garnotel E., Matan A. B., Gérôme P., Bernatas J. J., Buisson Y., Pourcel C. ( 2011). Clinical characteristics of the smooth tubercle bacilli ‘Mycobacterium canettii’ infection suggest the existence of an environmental reservoir. Clin Microbiol Infect 17:1013–1019 [View Article][PubMed]
    [Google Scholar]
  12. Kozlov V. S., Rotov V. I. ( 1977). [Isolation of Mycobacterium tuberculosis cultures from soil]. Probl Tuberk 9:78–80[PubMed]
    [Google Scholar]
  13. Little T. W., Naylor P. F., Wilesmith J. W. ( 1982). Laboratory study of Mycobacterium bovis infection in badgers and calves. Vet Rec 111:550–557[PubMed]
    [Google Scholar]
  14. Mba Medie F., Ben Salah I., Henrissat B., Raoult D., Drancourt M. ( 2011). Mycobacterium tuberculosis complex mycobacteria as amoeba-resistant organisms. PLoS ONE 6:e20499 [View Article][PubMed]
    [Google Scholar]
  15. Palmer M. V., Thacker T. C., Waters W. R., Gortázar C., Corner L. A. ( 2012). Mycobacterium bovis: a model pathogen at the interface of livestock, wildlife, and humans. Vet Med Int 2012:236205 [View Article][PubMed]
    [Google Scholar]
  16. Panteix G., Gutierrez M. C., Boschiroli M. L., Rouviere M., Plaidy A., Pressac D., Porcheret H., Chyderiotis G., Ponsada M. & other authors ( 2010). Pulmonary tuberculosis due to Mycobacterium microti: a study of six recent cases in France. J Med Microbiol 59:984–989 [View Article][PubMed]
    [Google Scholar]
  17. Salah I. B., Ghigo E., Drancourt M. ( 2009). Free-living amoebae, a training field for macrophage resistance of mycobacteria. Clin Microbiol Infect 15:894–905 [View Article][PubMed]
    [Google Scholar]
  18. Sweeney F. P., Courtenay O., Hibberd V., Hewinson R. G., Reilly L. A., Gaze W. H., Wellington E. M. ( 2007). Environmental monitoring of Mycobacterium bovis in badger feces and badger sett soil by real-time PCR, as confirmed by immunofluorescence, immunocapture, and cultivation. Appl Environ Microbiol 73:7471–7473 [View Article][PubMed]
    [Google Scholar]
  19. Tanner M., Michel A. L. ( 1999). Investigation of the viability of M. bovis under different environmental conditions in the Kruger National Park. Onderstepoort J Vet Res 66:185–190[PubMed]
    [Google Scholar]
  20. Thoen C. O., LoBue P. A. ( 2007). Mycobacterium bovis tuberculosis: forgotten, but not gone. Lancet 369:1236–1238 [View Article][PubMed]
    [Google Scholar]
  21. van Ingen J., Rahim Z., Mulder A., Boeree M. J., Simeone R., Brosch R., van Soolingen D. ( 2012). Characterization of Mycobacterium orygis as M. tuberculosis complex subspecies. Emerg Infect Dis 18:653–655 [View Article][PubMed]
    [Google Scholar]
  22. Van Soolingen D., Hoogenboezem T., de Haas P. E., Hermans P. W., Koedam M. A., Teppema K. S., Brennan P. J., Besra G. S., Portaels F. & other authors ( 1997). A novel pathogenic taxon of the Mycobacterium tuberculosis complex, Canetti: characterization of an exceptional isolate from Africa. Int J Syst Bacteriol 47:1236–1245 [View Article][PubMed]
    [Google Scholar]
  23. Walter W. D., Anderson C. W., Smith R., Vanderklok M., Averill J. J., Vercauteren K. C. ( 2012). On-farm mitigation of transmission of tuberculosis from white-tailed deer to cattle: literature review and recommendations. Vet Med Int 2012:616318 [View Article][PubMed]
    [Google Scholar]
  24. Young J. S., Gormley E., Wellington E. M. ( 2005). Molecular detection of Mycobacterium bovis and Mycobacterium bovis BCG (Pasteur) in soil. Appl Environ Microbiol 71:1946–1952 [View Article][PubMed]
    [Google Scholar]
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