1887

Abstract

While there is evidence for the persistence of in soil, there are no reports for the other complex (MTC) mycobacteria. Here, soil was inoculated with 10 c.f.u. g , and and subcultured monthly for 12 months. The pathogenicity of mycobacterial colonies, identified by using matrix-assisted laser desorption/ionization time of flight mass spectrometry, was assessed in a mouse model. Moreover, mice were fed with food that contained 16.7 % -contaminated soil. The three tested MTC species survived in soil for 12 months with a final inoculum of 2×10 c.f.u. g for , 150 c.f.u. g for and 2×10 c.f.u. g for . In an experiment that included negative controls, all (5/5) mice inoculated with such and developed 0.03–0.3 granulomas mm in their lungs and spleen and grew mycobacteria; five mice that were inoculated with from soil did not develop granulomas but grew mycobacteria. Furthermore, 0.2–0.4 granulomas mm were observed in the lungs and spleen of 3/5 mice fed with -contaminated soil in the presence of two negative control mice. grew in the stomach, intestine, spleen and lung in 5/5 challenged mice, whereas the negative controls remained -free ( = 0.008, Fisher exact test). This study provides clear evidence that MTC mycobacteria survive in soil, and that remains virulent while in the soil, outside its hosts, for extended periods of time.

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2014-03-01
2020-01-29
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