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Volume 160, Issue 1

Growth on mannitol-rich media elicits a genome-wide transcriptional response in that impacts on multiple virulence traits in an exopolysaccharide-independent manner Free

Abstract

In common with other members of the complex (BCC), is capable of producing exopolysaccharide (EPS) when grown on certain mannitol-rich media. The significance of the resulting mucoid phenotype and the genome-wide response to mannitol has never been characterized despite its clinical relevance following the approval of a dried-powder preparation of mannitol as an inhaled osmolyte therapy for cystic fibrosis (CF) patients. In the present study we defined the transcriptional response of ATCC 17616, a model genome-sequenced strain of environmental origin, to growth on mannitol-rich yeast extract media (MYEM). EPS-dependent and -independent impact of MYEM on virulence-associated traits was assessed in both strain ATCC 17616 and the CF isolate C1576. Our studies revealed a significant transcriptional response to MYEM encompassing approximately 23 % of predicted genes within the genome. Strikingly, this transcriptional response identified that EPS induction occurs in ATCC 17616 without the upregulation of the and EPS gene clusters, despite their pivotal role in EPS biosynthesis. Of approximately 20 differentially expressed putative virulence factors, 16 exhibited upregulation including flagella, ornibactin, oxidative stress proteins and phospholipases. MYEM-grown also exhibited enhanced motility, biofilm formation and epithelial cell invasion. In contrast to these potential virulence enhancements, MYEM-grown C1576 showed attenuated virulence in the infection model. All of the observed phenotypic responses occurred independently of EPS production, highlighting the profound impact that mannitol-based growth has on the physiology and virulence of .

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • CF Trust (Award PJ551 and RS29 )
  • US CF Foundation Therapeutics (Award MAHENT06V0)
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2014-01-01
2024-04-25
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Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner
Microbiology 160, 187 (2014); https://doi.org/10.1099/mic.0.072975-0
/content/journal/micro/10.1099/mic.0.072975-0
/content/journal/micro/10.1099/mic.0.072975-0
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