1887

Abstract

is often found as a member of the normal gut microflora in various animals. However, it has been reported to cause mastitis in cattle, septicaemia in pigeons, and meningitis, septicaemia and endocarditis in humans. However, little is known about the epidemiology and crucial virulence factors of . To help address these issues, we developed a multilocus sequence typing (MLST) scheme for . Seven housekeeping gene fragments were sequenced from each of 58 isolates collected from diverse origins and sources. The MLST scheme had good discriminatory ability. The 63 strains, including the 5 whole genome sequenced strains examined, resolved into 57 sequence types (STs), with 52 STs represented by only a single strain. With respect to the identification of subspecies (i.e. subsp. , subsp. and subsp. ), the results of biochemical tests and DNA–DNA hybridization were in high concordance with those of the MLST scheme. The MLST scheme developed in this study may be a useful tool capable of replacing the conventional methods used for subspecies identification. The results of this study suggest that the biology and virulence of two pathogenic subspecies (i.e. subsp. and subsp. ) are very different. The MLST scheme offers researchers a valuable typing tool that will promote further investigation of the epidemiology of .

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2014-01-01
2019-12-08
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