1887

Abstract

This study characterized the two-component regulatory systems encoded by and , and assessed their influence on biofilm formation by 100-23. A method for deletion of multiple genes was employed to disrupt the genetic loci of two-component systems. The operons and showed complementary organization. Genes encode a histidine kinase, a response regulator and an ATP-binding cassette-type transporter with a bacteriocin-processing peptidase domain, respectively. Genes code for a signal peptide, a histidine kinase and a response regulator, respectively. Deletion of single or multiple genes in the operons and did not affect cell morphology, growth or the sensitivity to various stressors. However, gene disruption affected biofilm formation; this effect was dependent on the carbon source. Deletion of or increased sucrose-dependent biofilm formation . Glucose-dependent biofilm formation was particularly increased by deletion of . The expression of and was altered by deletion of , indicating cross-talk between these two regulatory systems. These results may contribute to our understanding of the genetic factors related to the biofilm formation and competitiveness of in intestinal ecosystems.

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2014-04-01
2020-01-26
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