1887

Abstract

The gene encodes a quiescent porin that belongs to the OmpC/OmpF family. In the present work we analysed the regulatory effects of OmpR phosphorylation on expression. We found that , OmpR in its phosphorylated form (OmpR-P) was important in the regulation of the two promoters: OmpR-P activated the P1 promoter and repressed the P2 promoter in an EnvZ-dependent manner; expression occurs from the P2 promoter in an mutant. , OmpR-P had a higher DNA-binding-affinity to the promoter region than OmpR and OmpRD55A, showing an affinity even higher than that of equivalent DNA regions in the 5′-upstream regulatory sequence of the major porin-encoding genes and . By analysing different environmental conditions, we found that glucose and glycerol enhanced expression in the wild-type strain. Interestingly the stimulation by glycerol was OmpR-dependent while the effect of glucose was still observed in the absence of OmpR. Acetyl phosphate produced by the AckA-Pta pathway did not influence regulation. These data indicate the important role of the phosphorylation in the activity of OmpR on the differential regulation of both promoters and its impact on the pathogenesis.

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2014-04-01
2020-01-17
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