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Volume 159, Issue Pt_11

Insertion site and distribution of a genomic island conferring DNA phosphorothioation in the complex Free

Abstract

Nearly half of US clinical isolates of the emerging pathogen were reported to exhibit smeared DNA during PFGE. This DNA degradation (Dnd) phenotype results from DNA phosphorothioation, a sulfur modification found in other bacteria and conferred by genes located on mobile elements. Putative genes are located on a 19.6 kbp genomic island (GI) in the type strain ATCC 19977. We confirmed that ATCC 19977 is Dnd-positive by PFGE and we developed a PCR assay to predict Dnd phenotype. Dnd-positive strains generated an amplicon from whereas Dnd-negative strains generated a bridge amplicon that spanned the GI insertion site, indicating they lacked the entire ‘Dnd-GI’. Comparative analyses of sequences from the bridge amplicon with ATCC 19977 revealed the Dnd-GI is flanked by 22 bp repeats in and inserted downstream of a gene and between inverted repeats. Regions flanking the Dnd-GI were highly conserved within the complex. Bioinformatics studies suggest the Dnd-GI inserted independently into a strain of and that other species of mycobacteria also have genes, supporting reports that the Dnd phenotype is common among actinomycetes. Within the complex, Dnd-positive clinical isolates were primarily , and tandem repeat typing indicated these isolates were highly related, confirming previous PFGE studies and revealing a widespread family of strains with significance in human disease.

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2013-11-01
2024-04-23
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Insertion site and distribution of a genomic island conferring DNA phosphorothioation in the Mycobacterium abscessus complex
Microbiology 159, 2323 (2013); https://doi.org/10.1099/mic.0.070318-0
/content/journal/micro/10.1099/mic.0.070318-0
/content/journal/micro/10.1099/mic.0.070318-0
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