1887

Abstract

is a commensal of humans and an opportunistic pathogen. It can cause an aggressive form of infective endocarditis in healthy humans akin to . Here we compared the virulence of the genome-sequenced strain N920143 to in an experimental rat endocarditis model. N920143 caused a milder course of disease with lower levels of bacteraemia and smaller endocardial vegetations than strain Newman. However, vegetations were comparable to those produced by MRSA strain COL. Little is known about virulence factors of as systems to manipulate the bacterium genetically are currently limited. Here, we report a method for electroporation of with plasmid DNA and demonstrate that the low efficiency of transformation is due to the activity of a conserved type I restriction–modification system. To streamline the transformation process, we constructed SL01B, an strain expressing the / genes of N920143. Modified plasmid DNA isolated from SL01B transformed strains from clonal complexes 1 and 2 efficiently. A deletion mutant of N920143 lacking sortase A was significantly less virulent than the wild-type in the endocarditis model. Mutants defective in single surface proteins Fbl or vWbl were not significantly different from the wild-type but showed trends towards reduced virulence.

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2013-10-01
2019-11-14
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