1887

Abstract

A putative prenyltransferase gene, NFIA_043650, was amplified from NRRL 181 and cloned into the expression vector pQE60. The deduced polypeptide consisting of 445 amino acids with a molecular mass of 51 kDa was overproduced in and purified as His-tagged protein to near homogeneity. The purified soluble protein was subsequently assayed with potential aromatic substrates in the presence of dimethylallyl diphosphate. HPLC analysis of the reaction mixtures revealed acceptance of all tested tryptophan-containing cyclic dipeptides. Isolation and structural elucidation of enzyme products of five selected substrates indicated a reverse C2-prenylation on the indole nucleus, proving the enzyme to be a yclic ipetide -renylransferase (CdpC2PT). Differing significantly from two known brevianamide F reverse C2-prenyltransferases NotF and BrePT which use cyclo--Trp--Pro as their preferred substrate, CdpC2PT showed a clear substrate preference for ()-benzodiazepinedinone and cyclo--Trp--Trp with values of 84.1 and 165.2 µM and turnover numbers at 0.63 and 0.30 s, respectively. A possible role of CdpC2PT in the biosynthesis of fellutanines is discussed.

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2013-10-01
2019-10-19
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