1887

Abstract

(Sacc.) Nirenberg is among the most common species corn pathogens worldwide, and has been recognized as a fumonisin B (FB) and fumonisin B (FB) producer. In the present work, extracts of 58 isolates from corn samples collected from Sohag Governorate, Egypt, were tested for their biotoxicity and production of fumonisin toxins. Forty-four isolates out of 58 tested produced FB or FB and FB (15 and 29 isolates, respectively) on potato–sucrose agar medium, detected by TLC, whereas the other 14 isolates did not produce fumonisin toxins. HPLC crude extract analysis confirmed the results from TLC plates. Brine shrimp larvae as well as the Gram-negative bacteria showed low bio-sensitivity towards the crude extract toxicity, whereas the Gram-positive bacteria and , especially . , showed higher sensitivity towards the tested crude extracts. These results enabled us to bio-evaluate and chemically detect fumonisin mycotoxins using a simple agar medium technique.

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2013-08-01
2024-04-18
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