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Volume 159, Issue Pt_8

Potassium transport of is important for type III secretion and pathogenesis Free

Abstract

Intracellular cations are essential for the physiology of all living organisms including bacteria. Cations such as potassium ion (K), sodium ion (Na) and proton (H) are involved in nearly all aspects of bacterial growth and survival. K is the most abundant cation and its homeostasis in and is regulated by three major K transporters: high affinity transporter Kdp and low affinity transporters Kup and Trk. Previous studies have demonstrated the roles of cations and cation transport in the physiology of ; their roles in the virulence and physiology of pathogenic bacteria are not well characterized. We have previously reported that the K transporter Trk is important for the secretion of effector proteins of the type III secretion system (TTSS) of pathogenicity island 1 (SPI-1). Here we further explore the role of cation transport in virulence and pathogenesis in animal models. Impairment of K transport through deletion of K transporters or exposure to the chemical modulators of cation transport, gramicidin and valinomycin, results in a severe defect in the TTSS of SPI-1, and this defect in the TTSS was not due to a failure to regulate intrabacterial pH or ATP. Our results also show that K transporters are critical to the pathogenesis of in mice and chicks and are involved in multiple growth and virulence characteristics , including protein secretion, motility and invasion of epithelial cells. These results suggest that cation transport of the pathogenic bacterium , especially K transport, contributes to its virulence in addition to previously characterized roles in maintaining homeostasis of bacteria.

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2013-08-01
2024-04-19
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Potassium transport of Salmonella is important for type III secretion and pathogenesis
Microbiology 159, 1705 (2013); https://doi.org/10.1099/mic.0.068700-0
/content/journal/micro/10.1099/mic.0.068700-0
/content/journal/micro/10.1099/mic.0.068700-0
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