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Volume 159, Issue Pt_9

A new subgroup of the IS family and properties of its representative member IS Free

Abstract

Recently, we described a novel insertion element, IS, isolated from a permafrost strain of . In this work, we demonstrated that IS is a member of a novel subgroup of the IS family of insertion sequences (ISs) that was not identified and characterized previously. IS elements of this subgroup termed the IS subgroup are broadly distributed among different taxa of Eubacteria, including , , , , and Cyanobacteria. While displaying characteristic features of the IS-family elements, IS subgroup elements exhibit some unusual features. In particular, most of them have longer terminal repeats with unconventional ends and frameshifting box with an atypical organization, and, unlike many other IS-family elements, do not exhibit any distinct IS specificity. We studied the transposition and mutagenic properties of a representative member of this subgroup, IS and showed that in contrast to the original host, in a heterologous host, K-12, it is able to translocate with extremely high efficiency into the chromosome, either by itself or as a part of a composite transposon containing two IS copies. The majority of transposants carry multiple chromosomal copies (up to 12) of IS. It was discovered that IS is characterized by a marked mutagenic activity in : its chromosomal insertions generate various types of mutations, including auxotrophic, pleiotropic and rifampicin-resistance mutations. The distribution of IS elements of the novel subgroup among different bacteria, their role in the formation of composite transposons and the horizontal transfer of genes are examined and discussed.

  • Received:
  • Accepted:
  • Published Online:
© Microbiology Society
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2013-09-01
2024-04-23
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A new subgroup of the IS3 family and properties of its representative member ISPpy1
Microbiology 159, 1900 (2013); https://doi.org/10.1099/mic.0.068676-0
/content/journal/micro/10.1099/mic.0.068676-0
/content/journal/micro/10.1099/mic.0.068676-0
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