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Volume 159, Issue Pt_10

An gene sequence based evaluation of cultured diversity on different growth media Free

Abstract

The last decade has shown an increased interest in the utilization of bacteria for applications ranging from bioremediation to wastewater purification and promotion of plant growth. In order to extend the current number of micro-organism mediated applications, a continued quest for new agents is required. This study focused on the genus , which is known to harbour strains with a very diverse set of interesting properties. The aim was to identify growth media that allow retrieval of a high diversity, as such increasing the chance of isolating isolates with beneficial properties. Three cultivation media: trypticase soy agar (TSA), potato dextrose agar (PDA) and isolation agar (PIA) were evaluated for their abilities to grow strains. TSA and PDA were found to generate the largest diversity. However, communities obtained with both media overlapped. Communities obtained with PIA, on the other hand, were unique. This indicated that the largest diversity is obtained by sampling from either PDA or TSA and from PIA in parallel. To evaluate biodiversity of the isolated members on the media, an appropriate biomarker had to be identified. Hence, an introductory investigation of the taxonomic resolution of the 16S rRNA, , and genes was performed. The gene sequences not only had a high phylogenetic content and the highest taxonomic resolution amongst the genes investigated, it also had a gene phylogeny that related well with that of the 16S rRNA gene.

  • Received:
  • Accepted:
  • Published Online:
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2013-10-01
2024-04-25
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An rpoD gene sequence based evaluation of cultured Pseudomonas diversity on different growth media
Microbiology 159, 2097 (2013); https://doi.org/10.1099/mic.0.068031-0
/content/journal/micro/10.1099/mic.0.068031-0
/content/journal/micro/10.1099/mic.0.068031-0
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