1887

Abstract

CG43, a heavy encapsulated liver abscess isolate, mainly expresses type 3 fimbriae. Type 1 fimbriae expression was only apparent in CG43S3Δ (the type 3 fimbriae-deficient strain). The expression of type 1 fimbriae in CG43S3Δ was reduced by deleting the gene, but was unaffected by removing the 3′ end of encoding the C-terminal EIL domain (EIL). Quantitative RT-PCR and promoter activity analysis showed that the putative DNA-binding region at the N terminus, but not the C-terminal EIL domain, of FimK positively affects transcription of the type 1 fimbrial major subunit, . An electrophoretic mobility shift assay demonstrated that the recombinant FimK could specifically bind to , which is located upstream of and contains a vegetative promoter for the operon, also reflecting possible transcriptional regulation. EIL was shown to encode a functional phosphodiesterase (PDE) via enhancing motility in JM109 and using PDE activity assays. Moreover, EIL exhibited higher PDE activity than FimK, implying that the N-terminal DNA-binding domain may negatively affect the PDE activity of FimK. FimA expression was detected in CG43S3 expressing EIL or AIL, suggesting that FimA expression is not directly influenced by the c-di-GMP level. In summary, FimK influences type 1 fimbriation by binding to at the N-terminal domain, and thereafter, the altered protein structure may activate C-terminal PDE activity to reduce the intracellular c-di-GMP level.

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2013-07-01
2020-12-04
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