RT Journal Article SR Electronic(1) A1 Faure, Laura M. A1 Llamas, María A. A1 Bastiaansen, Karlijn C. A1 de Bentzmann, Sophie A1 Bigot, SarahYR 2013 T1 Phosphate starvation relayed by PhoB activates the expression of the Pseudomonas aeruginosa σvreI ECF factor and its target genes JF Microbiology, VO 159 IS Pt_7 SP 1315 OP 1327 DO https://doi.org/10.1099/mic.0.067645-0 PB Microbiology Society, SN 1465-2080, AB The cell-surface signalling (CSS) system represents an important regulatory mechanism by which Gram-negative bacteria respond to the environment. Gene regulation by CSS systems is particularly present and important in the opportunistic human pathogen Pseudomonas aeruginosa. In this bacterium, these mechanisms regulate mainly the uptake of iron, but also virulence functions. The latter is the case for the P. aeruginosa PUMA3 CSS system formed by the putative VreA receptor, the σVreI extracytoplasmic function sigma factor and the VreR anti-sigma factor. A role for this system in P. aeruginosa virulence has been demonstrated previously. However, the conditions under which this system is expressed and activated have not been elucidated so far. In this work, we have identified and characterized the global regulatory cascade activating the expression of the PUMA3 system. We show that the PhoB transcriptional regulator, part of the PhoB-PhoR two-component signalling system, can sense a limitation of inorganic phosphate to turn on the expression of the vreA, vreI and vreR genes, which constitute an operon. Upon expression of these genes in this condition, σVreI factor mediates transcription of most, but not all, of the previously identified σVreI-regulated genes. Indeed, we found new σVreI-targeted genes and we show that σVreI-regulon genes are all located immediately downstream to the vreAIR gene cluster., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.067645-0